Human Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) ELISA Kit | b4GALNT2 elisa kit

Human Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) ELISA Kit

Cat. Num. AAA2023037

• Gene Names: B4GALNT2; B4GALT; GALGT2
• Reactivity: Human
• Synonyms: Beta-1; 4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2); Human Beta-1; 4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) ELISA Kit; SD; B4GALGT2; B4GALT; Cad; GALGT2; Sda; Sd(a) beta-1; 4-GalNAc transferase; UDP-GalNAc:Neu5Aca2-3Galb-R b1; 4-N-acetylgalactosaminyltransferase; b4GALNT2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2).; No significant cross-reactivity or interference between Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) and analogues was observed.
• Sequence Length: 480

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: < 0.113ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for b4GALNT2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for b4GALNT2 elisa kit

Enzyme & Kinase

NCBI and Uniprot Product Information

• NCBI GI #: 109731790
• NCBI GeneID: 124872
• UniProt Accession #: Q8NHY0; Q14CP1; Q86Y40; B4DZE4
• Molecular Weight: 54,203 Da
• NCBI Official Full Name: Beta-1,4-N-acetyl-galactosaminyl transferase 2
• NCBI Official Synonym Full Names: beta-1,4-N-acetyl-galactosaminyl transferase 2
• NCBI Official Symbol: B4GALNT2
• NCBI Official Synonym Symbols: B4GALT; GALGT2
• NCBI Protein Information: beta-1,4 N-acetylgalactosaminyltransferase 2
• UniProt Protein Name: Beta-1,4 N-acetylgalactosaminyltransferase 2
• Protein Family: Beta-1,4 N-acetylgalactosaminyltransferase
• UniProt Gene Name: B4GALNT2
• UniProt Synonym Gene Names: GALGT2
• UniProt Entry Name: B4GN2_HUMAN

NCBI Description

B4GALNT2 catalyzes the last step in the biosynthesis of the human Sd(a) antigen through the addition of an N-acetylgalactosamine residue via a beta-1,4 linkage to a subterminal galactose residue substituted with an alpha-2,3-linked sialic acid. B4GALNT2 also catalyzes the last step in the biosynthesis of the Cad antigen (Montiel et al., 2003 [PubMed 12678917]).[supplied by OMIM, Mar 2008]

Uniprot Description

B4GALNT2: Involved in the synthesis of the Sd(a) antigen (Sia- alpha2,3-[GalNAc-beta1,4]Gal-beta1,4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain. Belongs to the glycosyltransferase 2 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Transferase; EC 2.4.1.-

Chromosomal Location of Human Ortholog: 17q21.32

Cellular Component: integral to membrane; integral to Golgi membrane

Molecular Function: acetylgalactosaminyltransferase activity

Biological Process: UDP-N-acetylglucosamine metabolic process; lipid glycosylation; protein amino acid glycosylation; negative regulation of cell-cell adhesion; UDP-N-acetylgalactosamine metabolic process