B3GNT1 Blocking Peptide | B3GNT1 (HGNC:15685) blocking peptide

B3GNT1 Antibody (C-term) Blocking Peptide

• Gene Names: B4GAT1; iGAT; iGNT; B3GNT1; B3GNT6; B3GN-T1; MDDGA13; BETA3GNTI
• Synonyms: B3GNT1; B3GNT1 Antibody (C-term) Blocking Peptide; Beta-1;4-glucuronyltransferase 1; 241-; I-beta-1;3-N-acetylglucosaminyltransferase; iGnT; N-acetyllactosaminide beta-1;3-N-acetylglucosaminyltransferase; Poly-N-acetyllactosamine extension enzyme; UDP-GlcNAc:betaGal beta-1;3-N-acetylglucosaminyltransferase 1; B3GNT1 (HGNC:15685) blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the C-term region of B3GNT1. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 415

• Cellular Location: Golgi apparatus membrane; Single-pass type II membrane protein. Note: Localizes near the trans-Golgi apparatus.
• Tissue Location: In the adult, highly expressed in heart, brain, skeletal muscle and kidney and to a lesser extent in placenta, pancreas, spleen, prostate, testis, ovary, small intestine and colon. Very weak expression in lung, liver, thymus and peripheral blood leukocytes. In fetal highly expressed in brain and kidney and to a lesser extent in lung and liver
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for B3GNT1 (HGNC:15685) blocking peptide

Beta-1,4-glucuronyltransferase involved in O- mannosylation of alpha-dystroglycan (DAG1). Transfers a glucuronic acid (GlcA) residue onto a xylose (Xyl) acceptor to produce the glucuronyl-beta-1,4-xylose-beta disaccharide primer, which is further elongated by LARGE, during synthesis of phosphorylated O- mannosyl glycan (PubMed:25279699, PubMed:25279697). Phosphorylated O-mannosyl glycan is a carbohydrate is a carbohydrate structure present in alpha-dystroglycan (DAG1), which is required for binding laminin G-like domain-containing extracellular proteins with high affinity (PubMed:25279699, PubMed:25279697). Required for axon guidance; via its function in O-mannosylation of alpha- dystroglycan (DAG1) (By similarity).

NCBI and Uniprot Product Information

• NCBI GI #: 23396461
• NCBI GeneID: 11041
• UniProt Accession #: O43505; Q4TTN0
• Molecular Weight: 47,119 Da
• NCBI Official Full Name: Beta-1,4-glucuronyltransferase 1
• NCBI Official Synonym Full Names: beta-1,4-glucuronyltransferase 1
• NCBI Official Symbol: B4GAT1
• NCBI Official Synonym Symbols: iGAT; iGNT; B3GNT1; B3GNT6; B3GN-T1; MDDGA13; BETA3GNTI
• NCBI Protein Information: beta-1,4-glucuronyltransferase 1
• UniProt Protein Name: Beta-1,4-glucuronyltransferase 1
• UniProt Gene Name: B4GAT1
• UniProt Synonym Gene Names: iGnT
• UniProt Entry Name: B4GA1_HUMAN

NCBI Description

This gene encodes a member of the beta-1,3-N-acetylglucosaminyltransferase family. This enzyme is a type II transmembrane protein. It is essential for the synthesis of poly-N-acetyllactosamine, a determinant for the blood group i antigen. [provided by RefSeq, Jul 2008]

Uniprot Description

B4GAT1: Can initiate the synthesis or the elongation of the linear poly-N-acetyllactosaminoglycans.

Protein type: Glycan Metabolism - glycosphingolipid biosynthesis - lacto and neolacto series; Transferase; EC 2.4.1.149; Glycan Metabolism - keratan sulfate biosynthesis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 11q13.2

Cellular Component: Golgi apparatus; Golgi membrane

Molecular Function: glucuronosyltransferase activity; N-acetyllactosaminide beta-1,3-N-acetylglucosaminyltransferase activity

Biological Process: keratan sulfate biosynthetic process; poly-N-acetyllactosamine biosynthetic process; protein amino acid O-linked mannosylation

Disease: Muscular Dystrophy-dystroglycanopathy (congenital With Brain And Eye Anomalies), Type A, 13