Human Actin Gamma 2, Smooth Muscle (ACTg2) ELISA Kit | ACTg2 elisa kit

Human Actin Gamma 2, Smooth Muscle (ACTg2) ELISA Kit

Cat. Num. AAA2703210

• Gene Names: ACTG2; ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• Reactivity: Human
• Synonyms: Actin Gamma 2; Smooth Muscle (ACTg2); Human Actin Gamma 2; Smooth Muscle (ACTg2) ELISA Kit; ACT; ACT-G2; ACTA3; ACTE; ACTL3; ACTSG; Alpha-actin-3; ACTg2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Actin Gamma 2, Smooth Muscle (ACTg2).; No significant cross-reactivity or interference between Actin Gamma 2, Smooth Muscle (ACTg2) and analogues was observed.
• Sequence Length: 333

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.053ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Actin Gamma 2, Smooth Muscle (ACTg2) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Actin Gamma 2, Smooth Muscle (ACTg2) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ACTg2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Actin Gamma 2, Smooth Muscle (ACTg2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Actin Gamma 2, Smooth Muscle (ACTg2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Actin Gamma 2, Smooth Muscle (ACTg2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 315570273
• NCBI GeneID: 72
• NCBI Accession #: NP_001186822.1
• NCBI GenBank Nucleotide #: NM_001199893.1
• UniProt Accession #: P63267; P12718; Q504R1; Q6FI22; B2R7E7; B4E315; D6W5H8; E9PG30
• Molecular Weight: 37,083 Da
• NCBI Official Full Name: actin, gamma-enteric smooth muscle isoform 2
• NCBI Official Synonym Full Names: actin, gamma 2, smooth muscle, enteric
• NCBI Official Symbol: ACTG2
• NCBI Official Synonym Symbols: ACT; ACTE; VSCM; ACTA3; ACTL3; ACTSG
• NCBI Protein Information: actin, gamma-enteric smooth muscle
• UniProt Protein Name: Actin, gamma-enteric smooth muscle
• Protein Family: Actin
• UniProt Gene Name: ACTG2
• UniProt Synonym Gene Names: ACTA3; ACTL3; ACTSG

NCBI Description

Actins are highly conserved proteins that are involved in various types of cell motility and in the maintenance of the cytoskeleton. Three types of actins, alpha, beta and gamma, have been identified in vertebrates. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. The beta and gamma actins co-exist in most cell types as components of the cytoskeleton and as mediators of internal cell motility. This gene encodes actin gamma 2; a smooth muscle actin found in enteric tissues. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Based on similarity to peptide cleavage of related actins, the mature protein of this gene is formed by removal of two N-terminal peptides.[provided by RefSeq, Dec 2010]

Uniprot Description

ACTG2: Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Belongs to the actin family.

Protein type: Contractile; Cytoskeletal; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: cytoplasm; cytosol; extracellular space; filopodium; lamellipodium

Molecular Function: ATP binding

Biological Process: muscle contraction

Disease: Visceral Myopathy