Mouse Azurocidin 1 ELISA Kit | AZU1 elisa kit

Mouse Azurocidin 1 ELISA Kit

Cat. Num. AAA724429

• Gene Names: AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• Reactivity: Mouse
• Synonyms: Azurocidin 1; Mouse Azurocidin 1 ELISA Kit; AZU1 elisa kit

• Reactivity: Mouse

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 1-25ng/mL
• Sensitivity: 0.1ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AZU1 elisa kit

Intended Uses: This AZU1 ELISA kit is intended Laboratory for research use only.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of AZU1 in the sample, this AZU1 ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus AZU1 concentration. The concentration of AZU1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Principle of the Assay: This AZU1 enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for AZU1. Standards or samples are then added to the microtiter plate wells and AZU1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of AZU1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for AZU1 are added to each well to "sandwich" the AZU1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain AZU1 and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.

NCBI and Uniprot Product Information

• NCBI GI #: 11342670
• NCBI GeneID: 566
• NCBI Accession #: NP_001691.1
• NCBI GenBank Nucleotide #: NM_001700.3
• Molecular Weight: 26,886 Da
• NCBI Official Full Name: azurocidin preproprotein
• NCBI Official Synonym Full Names: azurocidin 1
• NCBI Official Symbol: AZU1
• NCBI Official Synonym Symbols: AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR
• NCBI Protein Information: azurocidin; neutrophil azurocidin; heparin-binding protein; cationic antimicrobial protein 37; cationic antimicrobial protein CAP37
• UniProt Protein Name: Azurocidin
• Protein Family: Azurocidin
• UniProt Gene Name: AZU1
• UniProt Synonym Gene Names: HBP
• UniProt Entry Name: CAP7_HUMAN

NCBI Description

Azurophil granules, specialized lysosomes of the neutrophil, contain at least 10 proteins implicated in the killing of microorganisms. The protein encoded by this gene is an azurophil granule antibiotic protein, with monocyte chemotactic and antibacterial activity. It is also an important multifunctional inflammatory mediator. This encoded protein is a member of the serine protease gene family but it is not a serine proteinase, because the active site serine and histidine residues are replaced. The genes encoding this protein, neutrophil elastase 2, and proteinase 3 are in a cluster located at chromosome 19pter. All 3 genes are expressed coordinately and their protein products are packaged together into azurophil granules during neutrophil differentiation. [provided by RefSeq, Jul 2008]

Uniprot Description

AZU1: This is a neutrophil granule-derived antibacterial and monocyte- and fibroblast-specific chemotactic glycoprotein. Binds heparin. The cytotoxic action is limited to many species of Gram- negative bacteria; this specificity may be explained by a strong affinity of the very basic N-terminal half for the negatively charged lipopolysaccharides that are unique to the Gram-negative bacterial outer envelope. It may play a role in mediating recruitment of monocytes in the second wave of inflammation. Has antibacterial activity against the Gram-nagative bacterium P.aeruginosa, this activity is inhibited by LPS from P.aeruginosa. Acting alone, it does not have antimicrobial activity against the Gram-negative bacteria A.actinomycetemcomitans ATCC 29532, A.actinomycetemcomitans NCTC 9709, A.actinomycetemcomitans FDC-Y4, H.aphrophilus ATCC 13252, E.corrodens ATCC 23834, C.sputigena ATCC 33123, Capnocytophaga sp ATCC 33124, Capnocytophaga sp ATCC 27872 or E.coli ML-35. Has antibacterial activity against C.sputigena ATCC 33123 when acting synergistically with either elastase or cathepsin G. Belongs to the peptidase S1 family. Elastase subfamily.

Chromosomal Location of Human Ortholog: 19p13.3

Cellular Component: azurophil granule; extracellular region

Molecular Function: heparin binding; toxin binding; serine-type endopeptidase activity

Biological Process: positive regulation of tumor necrosis factor biosynthetic process; positive regulation of cell adhesion; microglial cell activation; glial cell migration; defense response to Gram-negative bacterium; proteolysis; macrophage chemotaxis; induction of positive chemotaxis; cellular extravasation; monocyte activation; positive regulation of MHC class II biosynthetic process; positive regulation of phagocytosis; positive regulation of fractalkine biosynthetic process; protein kinase C activation; positive regulation of interleukin-1 beta biosynthetic process; protein processing; immune response; regulation of vascular permeability; inflammatory response; negative regulation of apoptosis