Rat Vasopressin V1a receptor ELISA Kit | Avpr1a elisa kit

Rat Vasopressin V1a receptor ELISA Kit

Cat. Num. AAA760521

• Gene Names: AVPR1A; V1aR; AVPR1; AVPR V1a
• Reactivity: Rat
• Synonyms: Vasopressin V1a receptor; Rat Vasopressin V1a receptor ELISA Kit; Avpr1a/AVPR V1a/V1aR/Antidiuretic hormone receptor 1a/arginine vasopressin receptor 1A/AVPR V1a/AVPR1/SCCL vasopressin subtype 1a receptor/V1a vasopressin receptor/V1aR/V1-vascular vasopressin receptor AVPR1A/Vascular; hepatic-type arginine vasopressin receptor; Avpr1a elisa kit

• Reactivity: Rat
• Sequence Length: 418

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.469-30ug/ml
• Sensitivity: 0.281ug/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Avpr1a elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Rat Avpr1a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Rat Avpr1a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Rat Avpr1a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Rat Avpr1a can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4502331
• NCBI GeneID: 552
• NCBI Accession #: NP_000697.1
• NCBI GenBank Nucleotide #: NM_000706.4
• Molecular Weight: 46,800 Da
• NCBI Official Full Name: vasopressin V1a receptor
• NCBI Official Synonym Full Names: arginine vasopressin receptor 1A
• NCBI Official Symbol: AVPR1A
• NCBI Official Synonym Symbols: V1aR; AVPR1; AVPR V1a
• NCBI Protein Information: vasopressin V1a receptor
• UniProt Protein Name: Vasopressin V1a receptor
• Protein Family: Vasopressin V1a receptor
• UniProt Gene Name: AVPR1A
• UniProt Synonym Gene Names: AVPR1; V1aR
• UniProt Entry Name: V1AR_HUMAN

NCBI Description

The protein encoded by this gene acts as receptor for arginine vasopressin. This receptor belongs to the subfamily of G-protein coupled receptors which includes AVPR1B, V2R and OXT receptors. Its activity is mediated by G proteins which stimulate a phosphatidylinositol-calcium second messenger system. The receptor mediates cell contraction and proliferation, platelet aggregation, release of coagulation factor and glycogenolysis. [provided by RefSeq, Jul 2008]

Uniprot Description

AVPR1A: Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl- inositol-calcium second messenger system. Has been involved in social behaviors, including affiliation and attachment. Belongs to the G-protein coupled receptor 1 family. Vasopressin/oxytocin receptor subfamily.

Protein type: GPCR, family 1; Membrane protein, integral; Receptor, GPCR; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 12q14-q15

Cellular Component: cytoplasmic vesicle; cytosol; endosome; integral to plasma membrane; plasma membrane

Molecular Function: peptide binding; peptide hormone binding; protein binding; protein kinase C binding; V1A vasopressin receptor binding; vasopressin receptor activity

Biological Process: blood circulation; calcium-mediated signaling; cellular response to hormone stimulus; cellular response to water deprivation; elevation of cytosolic calcium ion concentration; G-protein coupled receptor protein signaling pathway; generation of precursor metabolites and energy; grooming behavior; maternal behavior; myotube differentiation; negative regulation of female receptivity; negative regulation of transmission of nerve impulse; penile erection; phospholipase C activation; positive regulation of cell growth; positive regulation of cell proliferation; positive regulation of cellular pH reduction; positive regulation of glutamate secretion; positive regulation of heart rate; positive regulation of prostaglandin biosynthetic process; positive regulation of systemic arterial blood pressure; positive regulation of vasoconstriction; regulation of systemic arterial blood pressure by vasopressin; response to corticosterone stimulus; social behavior; sperm ejaculation; telencephalon development