Bovine Attractin ELISA Kit | ATRN elisa kit

Bovine Attractin ELISA Kit

Cat. Num. AAA740989

• Gene Names: ATM; AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• Reactivity: Bovine
• Synonyms: Attractin; Bovine Attractin ELISA Kit; ATRN elisa kit

• Reactivity: Bovine

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Intended Uses: This ATRN ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Bovine ATRN. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATRN elisa kit

Principle of the assay: ATRN ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-ATRN antibody and an ATRN-HRP conjugate. The assay sample and buffer are incubated together with ATRN-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATRN concentration since ATRN from samples and ATRN-HRP conjugate compete for the anti-ATRN antibody binding site. Since the number of sites is limited, as more sites are occupied by ATRN from the sample, fewer sites are left to bind ATRN-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATRN concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATRN elisa kit

Neurobiology

NCBI and Uniprot Product Information

• NCBI GI #: 2304971
• NCBI GeneID: 472
• Molecular Weight: 350,687 Da
• NCBI Official Full Name: ATM
• NCBI Official Synonym Full Names: ATM serine/threonine kinase
• NCBI Official Symbol: ATM
• NCBI Official Synonym Symbols: AT1; ATA; ATC; ATD; ATE; ATDC; TEL1; TELO1
• NCBI Protein Information: serine-protein kinase ATM; AT mutated; A-T mutated; ataxia telangiectasia mutated; TEL1, telomere maintenance 1, homolog
• UniProt Protein Name: Serine-protein kinase ATM
• Protein Family: Attractin
• UniProt Gene Name: ATM
• UniProt Synonym Gene Names: A-T mutated
• UniProt Entry Name: ATM_HUMAN

NCBI Description

The protein encoded by this gene belongs to the PI3/PI4-kinase family. This protein is an important cell cycle checkpoint kinase that phosphorylates; thus, it functions as a regulator of a wide variety of downstream proteins, including tumor suppressor proteins p53 and BRCA1, checkpoint kinase CHK2, checkpoint proteins RAD17 and RAD9, and DNA repair protein NBS1. This protein and the closely related kinase ATR are thought to be master controllers of cell cycle checkpoint signaling pathways that are required for cell response to DNA damage and for genome stability. Mutations in this gene are associated with ataxia telangiectasia, an autosomal recessive disorder. [provided by RefSeq, Aug 2010]

Uniprot Description

ATM: an atypical kinase of the PIKK family. Regulates cell cycle checkpoints and DNA repair . May function as a tumor suppressor. Activates checkpoint signaling upon double strand breaks (DSBs), apoptosis and genotoxic stresses such as ionizing ultraviolet A light (UVA), thereby acting as a DNA damage sensor. Involved in the activation of ABL1 and SAPK. Binds DNA ends and is part of the BRCA1- associated genome surveillance complex (BASC), which contains BRCA1, MSH2, MSH6, MLH1, ATM, BLM, PMS2 and the RAD50-MRE11-NBN protein complex. This association could be a dynamic process changing throughout the cell cycle and within subnuclear domains. DNA damage promotes association with RAD17. LOF mutations associated with ataxia telangiectasia, causing progressive loss of motor control (ataxia), dilation of superficial blood vessels (telangiectasia), cancer and immune deficiency. Approximately 30% of cases develop tumors, mostly lymphomas and leukemias, due to defects in DNA damage repair. Somatic mutations seen in leukemias and lymphomas.

Protein type: Protein kinase, Ser/Thr (non-receptor); Kinase, protein; Tumor suppressor; EC 2.7.11.1; Protein kinase, atypical; DNA repair, damage; ATYPICAL group; PIKK family

Chromosomal Location of Human Ortholog: 11q22-q23

Cellular Component: nucleoplasm; chromosome, telomeric region; cytoplasmic membrane-bound vesicle; spindle

Molecular Function: protein dimerization activity; protein serine/threonine kinase activity; protein binding; DNA binding; 1-phosphatidylinositol-3-kinase activity; protein complex binding; DNA-dependent protein kinase activity; protein N-terminus binding; histone serine kinase activity; ATP binding

Biological Process: lipoprotein catabolic process; DNA damage induced protein phosphorylation; positive regulation of apoptosis; protein amino acid autophosphorylation; heart development; pre-B cell allelic exclusion; negative regulation of B cell proliferation; signal transduction; protein amino acid phosphorylation; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; positive regulation of neuron apoptosis; double-strand break repair; mitotic cell cycle spindle assembly checkpoint; cell cycle arrest; telomere maintenance; somitogenesis; V(D)J recombination; DNA repair; double-strand break repair via homologous recombination; peptidyl-serine phosphorylation; neuron apoptosis; DNA damage response, signal transduction resulting in induction of apoptosis; meiotic recombination; response to hypoxia; response to ionizing radiation; positive regulation of DNA damage response, signal transduction by p53 class mediator; brain development; response to DNA damage stimulus; oocyte development

Disease: Breast Cancer; Ataxia-telangiectasia