Western Blot (WB)
(Western BlotSample: Recombinant TNNI3, Human.)
Rabbit anti-Human Troponin I Type 3, Cardiac (TNNI3) Polyclonal Antibody | anti-TNNI3 antibody
Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3)
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Western Blot (WB)
(Western BlotSample: Recombinant TNNI3, Human.)
Western Blot (WB)
(Western BlotSample: Recombinant TNNI3, Human.)
Western Blot (WB)
(Western Blot;Sample:Lane 1: Rat Heart lysateLane 2: Rat Skeletal muscle lysateLane 3: Porcine Heart lysateLane 4: Porcine Skeletal muscle lysateLane 5: Mouse Heart lysateLane 6: Canine Heart lysateLane 7: Bovine Heart lysatePrimary Ab: 2ug/mL Rabbit Anti-Human TNNI3 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Western Blot (WB)
(Western Blot;Sample:Lane 1: Rat Heart lysateLane 2: Rat Skeletal muscle lysateLane 3: Porcine Heart lysateLane 4: Porcine Skeletal muscle lysateLane 5: Mouse Heart lysateLane 6: Canine Heart lysateLane 7: Bovine Heart lysatePrimary Ab: 2ug/mL Rabbit Anti-Human TNNI3 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-PSamples: Human Cardiac Muscle TissuePrimary Ab: 20ug/ml Rabbit Anti-Human TNNI3 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
Immunohistochemistry (IHC)
(DAB staining on IHC-PSamples: Human Cardiac Muscle TissuePrimary Ab: 20ug/ml Rabbit Anti-Human TNNI3 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Uniprot Description
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor
Chromosomal Location of Human Ortholog: 19q13.42
Cellular Component: cardiac Troponin complex; cytosol; sarcomere; troponin complex
Molecular Function: actin binding; actin filament binding; calcium channel inhibitor activity; calcium-dependent protein binding; metal ion binding; protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding
Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; muscle filament sliding; negative regulation of ATPase activity; regulation of cardiac muscle contraction by calcium ion signaling; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; vasculogenesis; ventricular cardiac muscle tissue morphogenesis
Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Dilated, 2a; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Familial Restrictive, 1
Research Articles on TNNI3
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Product Notes
The TNNI3 tnni3 (Catalog #AAA2004767) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Troponin I Type 3, Cardiac (TNNI3) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's Troponin I Type 3, Cardiac (TNNI3) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP) . Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. . Researchers should empirically determine the suitability of the TNNI3 tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Troponin I Type 3, Cardiac (TNNI3), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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