ATP1B3 recombinant protein

ATP1B3, 57- 279aa, Human, His tag, E Coli

Cat. Num. AAA205557

• Gene Names: ATP1B3; CD298; ATPB-3
• Applications: SDS-Page
• Purity: > 80% by SDS-PAGE
• Synonyms: ATP1B3; 57- 279aa; Human; His tag; E Coli; sodium/potassium-transporting ATPase subunit beta-3; ATPB-3; CD298; ATP1B3 recombinant protein

• Host: E Coli
• Purity/Purification: > 80% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 20% glycerol, 0.15M NaCl, 1mM DTT
• Concentration: 0.25mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSTMWVMLQ TLNDEVPKYR DQIPSPGLMV FPKPVTALEY TFSRSDPTSY AGYIEDLKKF LKPYTLEEQK NLTVCPDGAL FEQKGPVYVA CQFPISLLQA CSGMNDPDFG YSQGNPCILV KMNRIIGLKP EGVPRIDCVS KNEDIPNVAV YPHNGMIDLK YFPYYGKKLH VGYLQPLVAV QVSFAPNNTG KEVTVECKID GSANLKSQDD RDKFLGRVMF KITARA
• Sequence Length: 279

• Applicable Applications for ATP1B3 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ATP1B3 recombinant protein

ATP1B3 belongs to the X (+)/potassium ATPases subunit beta family. This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known. Recombinant human ATP1B3 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for ATP1B3 recombinant protein

Metabolism

References

Malik N., et al (1998). Mamm. Genome 9:136-143; Chi A., et al (2006). J. Proteome Res. 5:3135-3144;

NCBI and Uniprot Product Information

• NCBI GI #: 4502281
• NCBI GeneID: 483
• NCBI Accession #: NP_001670
• NCBI GenBank Nucleotide #: NM_001679.3
• UniProt Accession #: P54709; B7Z1N7
• Molecular Weight: 27.4 kDa (246aa) confirmed by MALDI-TOF
• NCBI Official Full Name: sodium/potassium-transporting ATPase subunit beta-3
• NCBI Official Synonym Full Names: ATPase Na+/K+ transporting subunit beta 3
• NCBI Official Symbol: ATP1B3
• NCBI Official Synonym Symbols: CD298; ATPB-3
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-3
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-3
• UniProt Gene Name: ATP1B3
• UniProt Synonym Gene Names: ATPB-3

NCBI Description

The protein encoded by this gene belongs to the family of Na+/K+ and H+/K+ ATPases beta chain proteins, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane. The glycoprotein subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes a beta 3 subunit. This gene encodes a beta 3 subunit. A pseudogene exists for this gene, and it is located on chromosome 2. [provided by RefSeq, Jul 2008]

Uniprot Description

This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The exact function of the beta-3 subunit is not known.