Ubiquitin-like-conjugating enzyme ATG3 Recombinant Protein | ATG3 recombinant protein

Recombinant Human Ubiquitin-like-conjugating enzyme ATG3

Cat. Num. AAA9420019

• Gene Names: ATG3; APG3; APG3L; PC3-96; APG3-LIKE
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: Ubiquitin-like-conjugating enzyme ATG3; Recombinant Human Ubiquitin-like-conjugating enzyme ATG3; Autophagy-related protein 3; APG3-like; hApg3; Protein PC3-96; ATG3 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Full Length, 1-314aa

• Tag Info: His-SUMO-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for ATG3 recombinant protein

E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt), autophagy, and mitochondrial homeostasis. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8-like proteins (GABARAP, GABARAPL1, GABARAPL2 or MAP1LC3A). The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8-like proteins from ATG3 to phosphatidylethanolamine (PE). This step is required for the mbrane association of ATG8-like proteins. The formation of the ATG8-phosphatidylethanolamine conjugates is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). Preferred substrate is MAP1LC3A. Also acts as an autocatalytic E2-like enzyme, catalyzing the conjugation of ATG12 to itself, ATG12 conjugation to ATG3 playing a role in mitochondrial homeostasis but not in autophagy. ATG7 (E1-like enzyme) facilitates this reaction by forming an E1-E2 complex with ATG3. Promotes primary ciliogenesis by roving OFD1 from centriolar satellites via the autophagic pathway.

NCBI and Uniprot Product Information

• NCBI GI #: 523704487
• NCBI GeneID: 64422
• NCBI Accession #: NP_001265641.1
• NCBI GenBank Nucleotide #: NM_001278712.1
• UniProt Accession #: Q9NT62; Q6PKC5; Q9H6L9
• Molecular Weight: 51.8kD
• NCBI Official Full Name: ubiquitin-like-conjugating enzyme ATG3 isoform 2
• NCBI Official Synonym Full Names: autophagy related 3
• NCBI Official Symbol: ATG3
• NCBI Official Synonym Symbols: APG3; APG3L; PC3-96; APG3-LIKE
• NCBI Protein Information: ubiquitin-like-conjugating enzyme ATG3
• UniProt Protein Name: Ubiquitin-like-conjugating enzyme ATG3
• Protein Family: Ubiquitin-like-conjugating enzyme
• UniProt Gene Name: ATG3
• UniProt Synonym Gene Names: APG3; APG3L; APG3-like; hApg3

NCBI Description

This gene encodes a ubiquitin-like-conjugating enzyme and is a component of ubiquitination-like systems involved in autophagy, the process of degradation, turnover and recycling of cytoplasmic constituents in eukaryotic cells. This protein is known to play a role in regulation of autophagy during cell death. A pseudogene of this gene is located on chromosome 20. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2013]

Uniprot Description

ATG3: an E2-like enzyme essential for Apg8p lipidation.

Protein type: Autophagy; EC 6.3.2.-; Ubiquitin conjugating system

Chromosomal Location of Human Ortholog: 3q13.2

Cellular Component: cytoplasmic ubiquitin ligase complex; cytosol

Molecular Function: APG12 conjugating enzyme activity; APG8 conjugating enzyme activity; enzyme binding; protein binding; small conjugating protein ligase activity

Biological Process: autophagic vacuole formation; macroautophagy; mitochondrial fragmentation during apoptosis; mitochondrion degradation; protein modification process; protein targeting to membrane; protein ubiquitination