Rabbit anti-Human NUDT15 Antibody | anti-NUDT15 antibody

NUDT15 Polyclonal Antibody

Cat. Num. AAA2541005

• Gene Names: NUDT15; MTH2; NUDT15D
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: NUDT15; NUDT15 Polyclonal Antibody; MTH2; NUDT15D; anti-NUDT15 antibody

• Host: Rabbit
• Reactivity: Human
• Isotype: IgG
• Purity/Purification: Affinity purification
• Form/Format: PBS with 0.02% sodium azide and 50% glycerol pH 7.4.

• Applicable Applications for anti-NUDT15 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:100
• Note: Centrifuge before opening to ensure complete recovery of vial contents.
• Immunogen: Recombinant protein of human NUDT15
• Preparation and Storage: Store at -20°C. Avoid freeze / thaw cycles.

Western Blot (WB)

(Western blot analysis of extracts of various cell lines with NUDT15 Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded human liver with NUDT15 Polyclonal Antibody)

Immunohistochemistry (IHC)

(Immunohistochemistry of paraffinembedded human stomach with NUDT15 Polyclonal Antibody)

Related Product Information for anti-NUDT15 antibody

This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified.

NCBI and Uniprot Product Information

• NCBI GI #: 754169469
• NCBI GeneID: 55270
• NCBI GenBank Nucleotide #: NM_001304745.1
• UniProt Accession #: Q9NV35; Q32Q27; Q6P2C9; A2RUR6
• Molecular Weight: Calculated MW: 18kDa; Observed MW: 19kDa
• NCBI Official Full Name: nucleotide triphosphate diphosphatase NUDT15 isoform 2
• NCBI Official Synonym Full Names: nudix hydrolase 15
• NCBI Official Symbol: NUDT15
• NCBI Official Synonym Symbols: MTH2; NUDT15D
• NCBI Protein Information: nucleotide triphosphate diphosphatase NUDT15
• UniProt Protein Name: Nucleotide triphosphate diphosphatase NUDT15
• Protein Family: Nudix hydrolase
• UniProt Gene Name: NUDT15
• UniProt Synonym Gene Names: Nudix hydrolase 15
• UniProt Entry Name: NUD15_HUMAN

NCBI Description

This gene encodes an enzyme that belongs to the Nudix hydrolase superfamily. Members of this superfamily catalyze the hydrolysis of nucleoside diphosphates, including substrates like 8-oxo-dGTP, which are a result of oxidative damage, and can induce base mispairing during DNA replication, causing transversions. The encoded enzyme is a negative regulator of thiopurine activation and toxicity. Mutations in this gene result in poor metabolism of thiopurines, and are associated with thiopurine-induced early leukopenia. Multiple pseudogenes of this gene have been identified. [provided by RefSeq, Apr 2016]

Uniprot Description

NUDT15: Mediates the hydrolysis of some nucleoside diphosphate derivatives. Can degrade 8-oxo-dGTP in vitro, suggesting that it may remove an oxidatively damaged form of guanine (7,8-dihydro-8- oxoguanine) from DNA and the nucleotide pool, thereby preventing misincorporation of 8-oxo-dGTP into DNA thus preventing A:T to C:G transversions. Its substrate specificity in vivo however remains unclear. May have a role in DNA synthesis and cell cycle progression through the interaction with PCNA. Belongs to the Nudix hydrolase family.

Protein type: EC 3.6.1.55; Hydrolase

Chromosomal Location of Human Ortholog: 13q14.2

Cellular Component: cytosol

Molecular Function: 8-oxo-7,8-dihydroguanine triphosphatase activity; nucleoside-diphosphatase activity; nucleoside-triphosphate diphosphatase activity; protein binding

Biological Process: dGTP catabolic process; DNA protection; exogenous drug catabolic process; mitotic cell cycle; purine nucleotide catabolic process

Disease: Thiopurines, Poor Metabolism Of, 2