Rat Activating Transcription Factor 3 ELISA Kit | ATF3 elisa kit

Rat Activating Transcription Factor 3 ELISA Kit

Cat. Num. AAA722565

• Reactivity: Rat
• Synonyms: Activating Transcription Factor 3; Rat Activating Transcription Factor 3 ELISA Kit; ATF3 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of ATF3. No significant cross-reactivity or interference between ATF3 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ATF3 and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 0.1 ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for ATF3 elisa kit

Intended Uses: This ATF3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of RatATF3. This ELISA kit for research use only, not for therapeutic or test applications!

Principle of the Assay: ATF3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-ATF3 antibody and an ATF3-HRP conjugate. The assay sample and buffer are incubated together with ATF3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ATF3 concentration since ATF3 from samples and ATF3-HRP conjugate compete for the anti-ATF3 antibody binding site. Since the number of sites is limited, as more sites are occupied by ATF3 from the sample, fewer sites are left to bind ATF3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ATF3 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for ATF3 elisa kit

Epigenetics and Nuclear Signaling

NCBI and Uniprot Product Information

• NCBI GI #: 47496615
• NCBI GeneID: 467
• UniProt Accession #: Q6ICQ9; Q5VTZ2; Q7Z566; Q8WYM6; P18847
• Molecular Weight: 14,314 Da
• NCBI Official Full Name: ATF3, partial
• NCBI Official Synonym Full Names: activating transcription factor 3
• NCBI Official Symbol: ATF3
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-3; cAMP-dependent transcription factor ATF-3
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-3
• Protein Family: Activating transcription factor
• UniProt Gene Name: ATF3
• UniProt Synonym Gene Names: cAMP-dependent transcription factor ATF-3
• UniProt Entry Name: ATF3_HUMAN

NCBI Description

This gene encodes a member of the mammalian activation transcription factor/cAMP responsive element-binding (CREB) protein family of transcription factors. This gene is induced by a variety of signals, including many of those encountered by cancer cells, and is involved in the complex process of cellular stress response. Multiple transcript variants encoding different isoforms have been found for this gene. It is possible that alternative splicing of this gene may be physiologically important in the regulation of target genes. [provided by RefSeq, Apr 2011]

Uniprot Description

ATF-3: This protein binds the cAMP response element (CRE) (consensus: 5'-GTGACGT[AC][AG]-3'), a sequence present in many viral and cellular promoters. Represses transcription from promoters with ATF sites. It may repress transcription by stabilizing the binding of inhibitory cofactors at the promoter. Isoform 2 activates transcription presumably by sequestering inhibitory cofactors away from the promoters. Belongs to the bZIP family. ATF subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor

Chromosomal Location of Human Ortholog: 1q32.3

Cellular Component: nucleoplasm; nucleolus; nucleus

Molecular Function: identical protein binding; protein binding; protein homodimerization activity; protein heterodimerization activity; transcription corepressor activity; transcription factor activity

Biological Process: cellular protein metabolic process; unfolded protein response, activation of signaling protein activity; transcription, DNA-dependent; unfolded protein response; positive regulation of cell proliferation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; gluconeogenesis