ATF2 recombinant protein

Recombinant Human ATF2 Protein (His & GST tag)

Cat. Num. AAA2545731

• Gene Names: ATF2; HB16; CREB2; TREB7; CREB-2; CRE-BP1
• Purity: > 90 % as determined by SDS-PAGE
• Synonyms: ATF2; Recombinant Human ATF2 Protein (His & GST tag); CRE-BP1; CREB2; HB16; MGC111558; TREB7; ATF2 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 90 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile 20mM Tris, 500mM NaCl, pH 8.0, 10% gly
• Sequence Length: 505

• Application Notes: The recombinant human ATF2/GST chimera consists of 742 amino acids and has a calculated molecular mass of 82.4 kDa. It migrates as an approximately 85 kDa band in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ATF2 recombinant protein

Background: Activating transcription factor 2, also known as ATF2, is a member of the leucine zipper family of DNA-binding proteins that binds to the cAMP response element. Its activity is enhanced after phosphorylation by stress-activated protein kinases such as c-Jun N-terminal kinase and p38. ATF2 has been found to be a target of the JNK signal transduction pathway and mediate adenovirus E1A-inducible transcriptional activation. ATF2 is also been reported playing roles in TGF-beta signaling pathway. It has been shown that the transcription factor ATF2 is bound by a hetero-oligomer of Smad3 and Smad4 upon TGF-beta stimulation. Studies indicate that ATF-2 plays a central role in TGF-beta signaling by acting as a common nuclear target of both Smad and TAK1 pathways. 

Description: A DNA sequence encoding full length of human ATF2 (P15336-1) (Met 1-Ser 505) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 368711269
• NCBI GeneID: 1386
• NCBI Accession #: NP_001243019.1
• NCBI GenBank Nucleotide #: NM_001256090.1
• UniProt Accession #: P15336; Q13000; Q3B7B7; Q4ZFU9; Q53RY2; Q8TAR1; A1L3Z2; A4D7U4; A4D7U5; A4D7V1; D3DPE9; G8JLM5
• Molecular Weight: 15,409 Da
• NCBI Official Full Name: cyclic AMP-dependent transcription factor ATF-2 isoform 1
• NCBI Official Synonym Full Names: activating transcription factor 2
• NCBI Official Symbol: ATF2
• NCBI Official Synonym Symbols: HB16; CREB2; TREB7; CREB-2; CRE-BP1
• NCBI Protein Information: cyclic AMP-dependent transcription factor ATF-2
• UniProt Protein Name: Cyclic AMP-dependent transcription factor ATF-2
• Protein Family: Alcohol O-acetyltransferase
• UniProt Gene Name: ATF2
• UniProt Synonym Gene Names: CREB2; CREBP1; cAMP-dependent transcription factor ATF-2; CREB-2; cAMP-responsive element-binding protein 2

NCBI Description

This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. The encoded protein has been identified as a moonlighting protein based on its ability to perform mechanistically distinct functions This protein binds to the cAMP-responsive element (CRE), an octameric palindrome. It forms a homodimer or a heterodimer with c-Jun and stimulates CRE-dependent transcription. This protein is also a histone acetyltransferase (HAT) that specifically acetylates histones H2B and H4 in vitro; thus it may represent a class of sequence-specific factors that activate transcription by direct effects on chromatin components. The encoded protein may also be involved in cell's DNA damage response independent of its role in transcriptional regulation. Several alternatively spliced transcript variants have been found for this gene [provided by RefSeq, Jan 2014]

Uniprot Description

ATF-2: a transcription factor that is a member of the leucine zipper family. Binds to the cAMP-responsive element (CRE). Forms a homodimer or heterodimer with c-Jun and stimulates CRE-dependent transcription. Also possesses histone acetyltransferase (HAT) activity that specifically acetylates histones H2B and H4 in vitro.

Protein type: C2H2-type zinc finger protein; EC 2.3.1.48; Transcription factor

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cytoplasm; mitochondrial outer membrane; nucleoplasm; nucleus

Molecular Function: cAMP response element binding protein binding; histone acetyltransferase activity; protein binding; protein kinase binding; RNA polymerase II transcription factor activity, enhancer binding; transcription coactivator activity; transcription factor activity

Biological Process: intra-S DNA damage checkpoint; positive regulation of transcription factor activity; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription factor activity; regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to DNA damage stimulus; response to osmotic stress