Factor XI Assay Kit

Human Factor XI total antigen ELISA assay

Cat. Num. AAA480386

• Synonyms: Factor XI; Human Factor XI total antigen ELISA assay; Hu. Coagulation Factor XI ELISA; Factor XI assay kit

• Sequence Length: 18

• Procedural Notes: 1. Use aseptic technique when opening and dispensing reagents.; 2. This kit is designed to work properly as provided and instructed. Additions, deletions, or substitutions to the procedure or reagents are not recommended, as they may be detrimental to the assay.; 3. Exercise universal precautions during the performance or handling of this kit or any component contained therein.
• Sample Collection and Preparation: The assay measures total human Factor XI in the 0.1-100 ng/ml range. Samples giving human Factor XI levels above 100 ng/ml should be diluted in Blocking Buffer before use. A 1:1,000 to 1:5,000 dilution for plasma is suggested for best results.
• Reagent Preparation: 1. 10x Wash Buffer: Dilute the 50 mL of concentrate to 1x with 450 mL of DI water prior to use.; 2. TBS Buffer: 0.1 M Tris, 0.15 M NaCl, pH 7.4.; 3. 3% BSA Blocking Buffer: 3% BSA in TBS Buffer.; 4. Standard: Reconstitute with DI Water as directed on the vial to give a 1000 ng/mL Standard Stock Solution. Prepare immediately prior to use.; 5. Primary Antibody: Reconstitute with 3% BSA Blocking Buffer as directed on the vial and vortex gently to mix. Prepare immediately prior to use.
• Standard Preparation: Prepare the Standard Stock Solution as described above. Do not prepare the standards until you are ready to apply them to the plate.
• Preparation and Storage: 1. Store this kit and its components at 4 degree C until use.; 2. The reconstituted Standard and Primary Antibody may be stored at -70 degree C for later use. DO NOT freeze/thaw the Standard or Primary Antibody more than once.

Testing Data

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Related Product Information for Factor XI assay kit

Introduction: This assay is intended for the quantitative determination of total Factor XI antigen in human plasma. Factor XI is a disulfide linked two-chain glycoprotein zymogen and is the precursor of the coagulation enzyme Factor XIa1. Factor XI consists of two identical monomers and circulates in plasma in complex with kininogen
2. Factor XI is activated by Factor XIIa and converts Factor IX to Factor IXa during the intrinsic pathway of the coagulation cascade3.

Principle of assay: Human Factor XI will bind to the affinity purified capture antibody coated on the microtiter plate. Factor XI and XIa will react with the antibody on the plate. After appropriate washing steps, peroxidase labeled polyclonal anti-human Factor XI primary antibody binds to the captured protein. Excess antibody is washed away and TMB substrate is used for color development at 450nm. A standard calibration curve is prepared along with the samples to be measured using dilutions of human Factor XI. Color development is proportional to the concentration of Factor XI in the samples.

References

1. Kurachi, K, and Davie, E.W.; (1981) Methods in Enzymology: 80: 211-220
2. Thompson, R.E., et al.; (1977) J. Clin, Invest.: 60: 1376
3. Walsh, P.N,; (1993) Methods in Enzymology: 222:65-96
4. Bouma, B.N., et al.; (1983) Blood: 62:1123-1131

NCBI and Uniprot Product Information

• NCBI GI #: 14626469
• Molecular Weight: 2,076 Da
• NCBI Official Full Name: factor XI, partial
• UniProt Protein Name: Factor XI
• Protein Family: Factor XIIa inhibitor
• UniProt Entry Name: Q96QS8_HUMAN