Testing Data
(Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).)
One-step TUNEL Assay Kit
One-step TUNEL Assay Kit (Green, AF488)
Synonyms
One-step TUNEL; One-step TUNEL Assay Kit (Green; AF488); One-step TUNEL assay kit
Preparation and Storage
Store at -20 degree C for 12 months. Label Solution should be stored in the dark.
Testing Data
(Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).)
Testing Data
(Paraffin embedded rat heart was treated with DNAse I to fragment the DNA. DNA strand breaks showed intense fluorescent staining in DNAse I treated sample (green). The cells were counterstained with DAPI (blue).)
Related Product Information for One-step TUNEL assay kit
When cells undergo apoptosis, specific DNA endonucleases will be activated, cutting the genomic DNA between the nucleosomes. After extraction and loading for electrophoresis, a DNA ladder of 180-200bp can be found in such DNA samples, which is a specific event during cell apoptosis. The exposed 3'-OH of the broken DNA can be catalyzed by Terminal Deoxynucleotidyl Transferase (TdT) with labeled dUTP, which can be detected with microscopy or flow cytometry.
One-step TUNEL Assay Kit is a highly sensitive, fast and simple method for cell apoptosis detection. The test results can be directly observed through a fluorescence microscope. This kit is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
One-step TUNEL Assay Kit is a highly sensitive, fast and simple method for cell apoptosis detection. The test results can be directly observed through a fluorescence microscope. This kit is suitable for in situ apoptosis detection of tissue samples (Paraffin embedding, freezing and ultrathin section) and cells (Cell smears, cell climbing smears) in situ apoptotic detection.
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Product Notes
The One-step TUNEL (Catalog #AAA2567762) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "One-step TUNEL, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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