Western Blot (WB)
(Western blot analysis of extracts from various samples, using DFFB Antibody.Lane 1: 293t, treated with blocking peptide;Lane 2: 293t;Lane 3: HepG2 cells.)
Rabbit DFFB Polyclonal Antibody | anti-DFFB antibody
DFFB Antibody
Predicted Reactivity: Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
Predicted Reactivity: Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%)
IF: 1:100-1:500
ICC: 1:100-1:500
IHC: 1:50-1:200
ELISA(peptide): 1:20,000-1:40,000
Western Blot (WB)
(Western blot analysis of extracts from various samples, using DFFB Antibody.Lane 1: 293t, treated with blocking peptide;Lane 2: 293t;Lane 3: HepG2 cells.)
Western Blot (WB)
(Western blot analysis of extracts from various samples, using DFFB Antibody.Lane 1: 293t, treated with blocking peptide;Lane 2: 293t;Lane 3: HepG2 cells.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612119 at 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunohistochemistry (IHC)-Paraffin
(MBS9612119 at 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612119 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612119) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Immunofluorescence (IF)/Immunocytochemistry (ICC)
(MBS9612119 staining HepG2 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(MBS9612119) and mouse anti-beta tubulin Ab(T0023) for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
Subcellular Location: Cytoplasm. Nucleus.
Subunit Structure: Heterodimer of DFFA and DFFB (PubMed:11371636). Interacts with H1-1 (PubMed:19882353).
NCBI and Uniprot Product Information
Predicted Molecular Weight: (Calculated)39kDa.
NCBI Description
Apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. The apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal DNA into nucleosomal units. DNA fragmentation factor (DFF) is a heterodimeric protein of 40-kD (DFFB) and 45-kD (DFFA) subunits. DFFA is the substrate for caspase-3 and triggers DNA fragmentation during apoptosis. DFF becomes activated when DFFA is cleaved by caspase-3. The cleaved fragments of DFFA dissociate from DFFB, the active component of DFF. DFFB has been found to trigger both DNA fragmentation and chromatin condensation during apoptosis. Alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of some of these variants has not been determined. [provided by RefSeq, Sep 2013]
Uniprot Description
DFFB: Nuclease that induces DNA fragmentation and chromatin condensation during apoptosis. Degrades naked DNA and induces apoptotic morphology. 4 isoforms of the human protein are produced by alternative splicing.
Protein type: Apoptosis; Deoxyribonuclease; EC 3.-.-.-
Chromosomal Location of Human Ortholog: 1p36.3
Cellular Component: nucleoplasm; nuclear chromatin; nucleolus; cytosol; nucleus
Molecular Function: enzyme binding; deoxyribonuclease activity
Biological Process: apoptosis; DNA fragmentation during apoptosis; apoptotic chromosome condensation; cell structure disassembly during apoptosis
Research Articles on DFFB
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Product Notes
The DFFB dffb (Catalog #AAA9612119) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The DFFB Antibody reacts with Human, Mouse, Rat Predicted Reactivity: Pig(88%), Zebrafish(83%), Bovine(88%), Horse(88%), Sheep(88%), Dog(88%) and may cross-react with other species as described in the data sheet. AAA Biotech's DFFB can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA). WB: 1:500-1:2000 IF: 1:100-1:500 ICC: 1:100-1:500 IHC: 1:50-1:200 ELISA(peptide): 1:20,000-1:40,000. Researchers should empirically determine the suitability of the DFFB dffb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DFFB, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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