Testing Data
cADP-Ribose Assay Kit | cADP assay kit
cADP-Ribose Assay Kit (Fluorescence)
Synonyms
cADP-Ribose; cADP-Ribose Assay Kit (Fluorescence); cADP assay kit
Application Notes
The assay can be conveniently performed in a 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 420/480 nm.
Detection
Fluorescence microplate reader
Kit Components
Component A: NAD Fluor Dye 1 bottle (5 ml)
Component B: ADPRC Enzyme Mix 1 vial
Component C: Assay Buffer I 1 bottle (12 ml)
Component D: Assay Buffer II 1 bottle (5 ml)
Component E: Enhancer Solution 1 bottle (3.5 ml)
Component F: cADPR Standard 1 vial
Component B: ADPRC Enzyme Mix 1 vial
Component C: Assay Buffer I 1 bottle (12 ml)
Component D: Assay Buffer II 1 bottle (5 ml)
Component E: Enhancer Solution 1 bottle (3.5 ml)
Component F: cADPR Standard 1 vial
Preparation and Storage
Keep in freezer (-20 degree C) and avoid exposure to light. All reagents are stable for at least 6 months after receipt when stored properly at the recommended conditions.
Testing Data
Testing Data
Testing Data
(Figure 1. The concentration of cADPR was detected using EliteTM cADP-ribose Assay Kit. Different concentrations ofcADPR were incubated with ADPRC reaction mix for 20 min at RT before NAD detection reagent was added. The lowestdetected concentration of cADPR is 100 nM.)
Testing Data
(Figure 1. The concentration of cADPR was detected using EliteTM cADP-ribose Assay Kit. Different concentrations ofcADPR were incubated with ADPRC reaction mix for 20 min at RT before NAD detection reagent was added. The lowestdetected concentration of cADPR is 100 nM.)
Related Product Information for cADP assay kit
Description: cADP-ribose (cADPR) is a novel Ca2+ messenger derived from NAD+. ADP-riboxyl cyclase (ADPRC) catalyzes thesynthesis of cADPR from NAD+, but the reaction can be reversed in the presence of high concentration of nicotinamide,producing NAD+from cADPR stoichiometrically. The resultant NAD+ can be detected using our newly developed NADsensor NAD Fluor Dye. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range.The NAD+ detection using NAD Fluor Dye is specific to NAD+ and has no reaction to NADH. The fluorescent signal can bereadily detected at Ex/Em = 420/480 nm. This assay can be performed in a convenient 96-well or 384-well microtiter plate.
The Elite cADP-ribose Assay Kit provides an ultrasensitive assay to quantify NAD in the sample. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range.
The Elite cADP-ribose Assay Kit provides an ultrasensitive assay to quantify NAD in the sample. This makes monitoring cADPR in tissues and cell cultures possible in the low nM range.
Product Categories/Family for cADP assay kit
Similar Products
Product Notes
The cADP (Catalog #AAA430588) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. The assay can be conveniently performed in a 96-well or 384-well microtiter-plate format. Its signal can be easily read by a fluorescence microplate reader at Ex/Em = 420/480 nm. Researchers should empirically determine the suitability of the cADP for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "cADP-Ribose, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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