Testing Data
(Jurkat cells were treated with 0.5 uM Rapamycin and 10 uM Chloroquine (red histogram) to induce autophagy or were mock treated (black histogram) for 18 hours. Cells were stained with Autophagy Probe, Red for 1 hour at 37 degree C, washed, and then analyzed using a BD Fortessa Special Order Flow Cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter. Median fluorescence intensity was 3-fold greater in induced cells (10,530) compared to mock-treated cells (3, 497). Data courtesy of Dr. Strandberg, ICT, 228:16-20. )
Autophagy Assay Kit
Autophagy Assay Kit, Red
Synonyms
Autophagy; Autophagy Assay Kit; Red; Autophagy assay kit
Samples
Cell culture
Excitation/Emission
590 nm / 620 nm
Reagent
Autophagy probe
Preparation and Storage
Store at <= -20 degree C for Nigericin component, 2-8 degree C for remaining kit components.
Testing Data
(Jurkat cells were treated with 0.5 uM Rapamycin and 10 uM Chloroquine (red histogram) to induce autophagy or were mock treated (black histogram) for 18 hours. Cells were stained with Autophagy Probe, Red for 1 hour at 37 degree C, washed, and then analyzed using a BD Fortessa Special Order Flow Cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter. Median fluorescence intensity was 3-fold greater in induced cells (10,530) compared to mock-treated cells (3, 497). Data courtesy of Dr. Strandberg, ICT, 228:16-20. )
Testing Data
(Jurkat cells were treated with 0.5 uM Rapamycin and 10 uM Chloroquine (red histogram) to induce autophagy or were mock treated (black histogram) for 18 hours. Cells were stained with Autophagy Probe, Red for 1 hour at 37 degree C, washed, and then analyzed using a BD Fortessa Special Order Flow Cytometer equipped with a green/yellow laser (561 nm excitation) and a 610/20 emission filter. Median fluorescence intensity was 3-fold greater in induced cells (10,530) compared to mock-treated cells (3, 497). Data courtesy of Dr. Strandberg, ICT, 228:16-20. )
Related Product Information for Autophagy assay kit
Description: The Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer.
Background: The Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer. Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications in cancer, infection, and degenerative diseases. Autophagy is a three stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs.
Background: The Autophagy Assay, Red enables researchers to detect and monitor the in vitro development of autophagy in living cells. The Autophagy Probe is cell-permeant and fluoresces red when inserted in the lipid membranes of autophagosomes and autolysosomes. Results can be read using a flow cytometer. Autophagy is a conserved lysosomal recycling process by which cells break down their own components such as proteins, lipids, and carbohydrates. The process plays an important role in maintaining homeostasis and destroying intracellular pathogens. In addition, autophagy can be upregulated in times of starvation or stress to provide additional nutrients for the cell. Dysregulation of autophagy has implications in cancer, infection, and degenerative diseases. Autophagy is a three stage process. First, cytoplasmic components targeted for degradation are sequestered, resulting in the formation of the autophagosome. Next, the autophagosome fuses with the lysosome to form the autophagolysosome or autolysosome. Finally, degradation of the autophagosomal contents occurs.
Product Categories/Family for Autophagy assay kit
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Product Notes
The Autophagy (Catalog #AAA258051) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Autophagy, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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