Dot Blot (WB)
(Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations)
ANNEXIN V Assay Kit | ANXA5 assay kit
ANNEXIN V: FITC ASSAY KIT
.2) Wash cells in PBS by gentle shaking or pipetting up and down.
3) Resuspend cells in 200ul pre-diluted binding buffer, adjusting to a cell density of 2-5 x 105 cells/ml
4) Add 5ul Annexin V:Biotin to 195ul of the cell suspension prepared in step 3
5) Mix and incubate for 15 minutes at room temperature.
6) Wash cells twice in 190ul of pre-diluted binding buffer.
7) Resuspend cells in 190ul pre-diluted binding buffer.
8) Add streptavidin:FITC conjugate.
9) Mix and incubate for 30 minutes in the dark, at room temperature.
10) Wash cells in 200ul pre-diluted binding buffer.
11) Resuspend cells in 190ul pre-diluted binding buffer.
12) Add 10ul of the Propidium Iodide solution.
13) Analyse by flow cytometry.
The flow cytometer is preferably set such that the Mean Fluorescence Intensity of the Annexin V negative population is between 1 and 10. Optimal parameter settings can be found using a positive control. For a positive control, incubate the cells with 3% formaldehyde in buffer during 30 minutes on ice. Wash away the formaldehyde and suspend the cells in cold binding buffer at 2-5 x 105 cells/ml. Proceed with step 2 as described above.
Shelf Life: 12 months from date of despatch
Dot Blot (WB)
(Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations)
Dot Blot (WB)
(Dot-plot showing Ramos cells staining with Annexin V:FITC (ANNEX100F) versus Propidium Iodide resulting in three distinct populations)
Testing Data
(Published customer image: Annexin V:FITC Assay Kit used for cell cycle analysis of cells treated with Arsenic trioxide and Macuna macrocarpa extract by flow cytometry.Image caption:Annexin V-FITC/propidium iodide (PI) analyses of arsenic trioxide (ATO)- and/or crude methanolic extract of Mucuna macrocarpa (CMEMM)-treated cells. HL-60 (a) and Jurkat (b) cells (1 x 105?cells/mL) were first treated with 5?mM N-acetyl cysteine (NAC), 50?μM butylated hydroxytoluene (BHT), or 40?μM alpha-tocopherol (VitE) or untreated, followed by treatment with 0.1% DMSO (CTL), 2.5?μM ATO, and/or 50?μg/mL CMEMM as indicated for 24?h. Quantitative percentages of apoptotic cells of ATO/CMEMM-treated cells were measured by flow cytometry. Data represent the result from one of three independent experiments.From: Lu KH, Lee HJ, Huang ML, Lai SC, Ho YL, Chang YS, Chi CW. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism.Evid Based Complement Alternat Med. 2012;2012:921430.)
Testing Data
(Published customer image: Annexin V:FITC Assay Kit used for cell cycle analysis of cells treated with Arsenic trioxide and Macuna macrocarpa extract by flow cytometry.Image caption:Annexin V-FITC/propidium iodide (PI) analyses of arsenic trioxide (ATO)- and/or crude methanolic extract of Mucuna macrocarpa (CMEMM)-treated cells. HL-60 (a) and Jurkat (b) cells (1 x 105?cells/mL) were first treated with 5?mM N-acetyl cysteine (NAC), 50?μM butylated hydroxytoluene (BHT), or 40?μM alpha-tocopherol (VitE) or untreated, followed by treatment with 0.1% DMSO (CTL), 2.5?μM ATO, and/or 50?μg/mL CMEMM as indicated for 24?h. Quantitative percentages of apoptotic cells of ATO/CMEMM-treated cells were measured by flow cytometry. Data represent the result from one of three independent experiments.From: Lu KH, Lee HJ, Huang ML, Lai SC, Ho YL, Chang YS, Chi CW. Synergistic Apoptosis-Inducing Antileukemic Effects of Arsenic Trioxide and Mucuna macrocarpa Stem Extract in Human Leukemic Cells via a Reactive Oxygen Species-Dependent Mechanism.Evid Based Complement Alternat Med. 2012;2012:921430.)
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa. [provided by RefSeq, Jul 2008]
Uniprot Description
This protein is an anticoagulant protein that acts as an indirect inhibitor of the thromboplastin-specific complex, which is involved in the blood coagulation cascade.
Research Articles on ANXA5
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Product Notes
The ANXA5 anxa5 (Catalog #AAA224962) is an Assay Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's ANNEXIN V can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the ANXA5 anxa5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ANNEXIN V, Assay Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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