Typical Testing Data/Standard Curve (for reference only)
Rat Acylation Stimulating Protein ELISA Kit | ASP elisa kit
Rat Acylation Stimulating Protein ELISA Kit
Gene Names
CPAMD8; VIP; K-CAP
Reactivity
Rat
Synonyms
Acylation Stimulating Protein; Rat Acylation Stimulating Protein ELISA Kit; ASP elisa kit
Reactivity
Rat
Samples
Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
Assay Type
Sandwich
Detection Range
250-5000pg/mL
Sensitivity
1.0pg/ml
Intended Uses
This ASP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ASP. This ELISA kit for research use only, not for therapeutic applications!
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for ASP elisa kit
For samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Intended UseThis ASP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ASP. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ASP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASP. Standards or samples are then added to the microtiter plate wells and ASP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASP are added to each well to “sandwich” the ASP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASP concentration in each sample is interpolated from this standard curve
Intended UseThis ASP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rat ASP. This ELISA kit for research use only!
PRINCIPLE OF THE ASSAY ASP ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for ASP. Standards or samples are then added to the microtiter plate wells and ASP if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of ASP present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for ASP are added to each well to “sandwich” the ASP immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain ASP and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ASP concentration in each sample is interpolated from this standard curve
Product Categories/Family for ASP elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
199,171 Da
NCBI Official Full Name
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
NCBI Official Synonym Full Names
C3 and PZP-like, alpha-2-macroglobulin domain containing 8
NCBI Official Symbol
CPAMD8
NCBI Official Synonym Symbols
VIP; K-CAP
NCBI Protein Information
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8; alpha-2 macroglobulin family protein VIP
UniProt Protein Name
C3 and PZP-like alpha-2-macroglobulin domain-containing protein 8
Protein Family
UniProt Gene Name
CPAMD8
UniProt Synonym Gene Names
KIAA1283
UniProt Entry Name
CPMD8_HUMAN
NCBI Description
CPAMD8 belongs to the complement component-3 (C3; MIM 120700)/alpha-2-macroglobulin (A2M; MIM 103950) family of proteins, which are involved in innate immunity and damage control. Complement components recognize and eliminate pathogens by direct binding or by mediating opsonization/phagocytosis and intracellular killing, and A2M is a broad-spectrum protease inhibitor (Li et al., 2004 [PubMed 15177561]).[supplied by OMIM, Mar 2008]
Uniprot Description
CPAMD8: Belongs to the protease inhibitor I39 (alpha-2- macroglobulin) family. Up-regulated by IL1B/interleukin-1 beta and IL6/interleukin-6. Interacts with heparin. 2 isoforms of the human protein are produced by alternative splicing
Chromosomal Location of Human Ortholog: 19p13.11
Cellular Component: extracellular space; plasma membrane
Molecular Function: serine-type endopeptidase inhibitor activity
Research Articles on ASP
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Product Notes
The Rat ASP cpamd8 (Catalog #AAA728340) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA728340 ELISA Kit recognizes Rat ASP. It is sometimes possible for the material contained within the vial of "Acylation Stimulating Protein, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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