Histone-lysine N-methyltransferase ASHH2 (ASHH2) Recombinant Protein | ASHH2 recombinant protein

Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2 (ASHH2) , partial

• Gene Names: EFS; ASH1 HOMOLOG 2; ASHH2; CAROTENOID CHLOROPLAST REGULATORY1; CCR1; EARLY FLOWERING IN SHORT DAYS; LAZ2; LAZARUS 2; SDG8; SET DOMAIN GROUP 8; T14N5.15; T14N5_15
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Histone-lysine N-methyltransferase ASHH2 (ASHH2); Recombinant Arabidopsis thaliana Histone-lysine N-methyltransferase ASHH2 (ASHH2); partial; ASHH2 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 1,805

• Species: Arabidopsis thaliana (Mouse-ear cress)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 240254387
• NCBI GeneID: 844066
• NCBI Accession #: NP_177854.6
• NCBI GenBank Nucleotide #: NM_106379.9
• UniProt Accession #: Q2LAE1; O80663; Q56WW4; A9QA57
• Molecular Weight: 198,851 Da
• NCBI Official Full Name: histone-lysine N-methyltransferase
• NCBI Official Symbol: EFS
• NCBI Official Synonym Symbols: ASH1 HOMOLOG 2; ASHH2; CAROTENOID CHLOROPLAST REGULATORY1; CCR1; EARLY FLOWERING IN SHORT DAYS; LAZ2; LAZARUS 2; SDG8; SET DOMAIN GROUP 8; T14N5.15; T14N5_15
• NCBI Protein Information: histone-lysine N-methyltransferase
• UniProt Protein Name: Histone-lysine N-methyltransferase ASHH2
• Protein Family: Histone-lysine N-methyltransferase
• UniProt Gene Name: ASHH2
• UniProt Synonym Gene Names: H3-K36-HMTase 8

NCBI Description

Encodes a protein with histone lysine N-methyltransferase activity required specifically for the trimethylation of H3-K4 in FLC chromatin (and not in H3-K36 dimethylation). Acts as an inhibitor of flowering specifically involved in the autonomous promotion pathway. EFS also regulates the expression of genes involved in carotenoid biosynthesis.Modification of histone methylation at the CRTISO locus reduces transcript levels 90%. The increased shoot branching seen in some EFS mutants is likely due to the carotenoid biosynthesis defect having an effect on stringolactones.Required for ovule, embryo sac, anther and pollen development.

Uniprot Description

Histone methyltransferase involved in di and tri-methylation of 'Lys-36' of histone H3 (H3K36me2 and H3K36me3). Binds to H3 already mono- or di-methylated on 'Lys-4'(H3K4me1 or H3K4me2), but not to H3K4me3. H3K4me and H3K36me represent specific tags for epigenetic transcriptional activation. Regulates positively FLC transcription to prevent early flowering transition. Required for flowering transition in response to vernalization and for the maintenance of FLC expression in late embryos, but dispensable for the initial reactivation in early embryos during reprogramming. Seems also to modulate several traits including floral organ size, root size and dormancy. Promotes apical dominance (PubMed:16299497, PubMed:10518493, PubMed:16258034, PubMed:18070919, PubMed:19915673, PubMed:20711170). Directly involved in the tri-methylation of 'Lys-36' of histone H3 (H3K36me3) at LAZ5 chromatin to maintain a transcriptionally active state of LAZ5, a TIR-NB-LRR protein involved in innate immunity (PubMed:20949080). Required for brassinosteroid (BR)-induced gene expression and histone H3 trimethylation on 'Lys-36' (H3K36me3) in BR-regulated genes (PubMed:24838002).