ASAH2 recombinant protein

Recombinant Mouse ASAH2 Protein (His tag)

Cat. Num. AAA2547219

• Gene Names: Asah2; AI585898
• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: ASAH2; Recombinant Mouse ASAH2 Protein (His tag); AI585898; Asah2; ASAH2 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4
• Sequence Length: 756

• Application Notes: The secreted recombinant mouse ASAH2 consists of 739 amino acids and has a calculated molecular mass of 82 kDa. As a result of glycosylation, the recombinant protein migrates as an approximately 105-115 kDa protein in SDS-PAGE under reducing conditions.
• Predicted N Terminal: His
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for ASAH2 recombinant protein

Background: ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses 'amucin box', a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

Description: A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal polyhistidine tag.

NCBI and Uniprot Product Information

• NCBI GI #: 9055168
• NCBI GeneID: 54447
• NCBI Accession #: NP_061300.1
• NCBI GenBank Nucleotide #: NM_018830.1
• UniProt Accession #: Q9JHE3; Q3UWP9; Q8BNP0; Q8BQN7; Q8R236
• Molecular Weight: 83,509 Da
• NCBI Official Full Name: neutral ceramidase
• NCBI Official Synonym Full Names: N-acylsphingosine amidohydrolase 2
• NCBI Official Symbol: Asah2
• NCBI Official Synonym Symbols: AI585898
• NCBI Protein Information: neutral ceramidase
• UniProt Protein Name: Neutral ceramidase
• Protein Family: Neutral ceramidase
• UniProt Gene Name: Asah2
• UniProt Synonym Gene Names: N-CDase; NCDase

Uniprot Description

ASAH2: Hydrolyzes the sphingolipid ceramide into sphingosine and free fatty acid at an optimal pH of 6.5-8.5. Acts as a key regulator of sphingolipid signaling metabolites by generating sphingosine at the cell surface. Acts as a repressor of apoptosis both by reducing C16-ceramide, thereby preventing ceramide-induced apoptosis, and generating sphingosine, a precursor of the antiapoptotic factor sphingosine 1-phosphate. Probably involved in the digestion of dietary sphingolipids in intestine by acting as a key enzyme for the catabolism of dietary sphingolipids and regulating the levels of bioactive sphingolipid metabolites in the intestinal tract. Belongs to the neutral ceramidase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.5.1.23; Hydrolase; Lipid Metabolism - sphingolipid; Membrane protein, integral

Chromosomal Location of Human Ortholog: 19|19 C1

Cellular Component: membrane; mitochondrion

Molecular Function: ceramidase activity

Biological Process: cell proliferation; response to organic substance