ASAH2 active protein

ASAH2 Protein, Mouse, Recombinant (His Tag)

Cat. Num. AAA8122456

• Gene Names: Asah2; AI585898
• Purity: >97% as determined by SDS-PAGE
• Synonyms: ASAH2; ASAH2 Protein; Mouse; Recombinant (His Tag); Mouse ASAH2 Protein (His Tag); AI585898 Protein; N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2; ASAH2 active protein

• Host: HEK293 Cells
• Purity/Purification: >97% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Thr34-Thr756

• Species: Mouse
• Activity: Measured by its ability to hydrolyze the substrate C12:0 ceramide into sphingosine and dodecanoic acid. The specific activity is >3,000pmoles/min/ug.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: His
• Tag: N-His
• Protein Construction: A DNA sequence encoding the Lumenal domain (Thr 34-Thr 756) of mouse ASAH2 (NP_061300.1) precursor was expressed with a N-terminal polyhistidine tag.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for ASAH2 active protein

Background: ASAH2 (N-acylsphingosine amidohydrolase 2), also known as neutral ceramidase, is a type II integral membrane protein that can be cleaved to produce a soluble secreted protein. The enzyme is abundant in the brush border membranes of the intestine, and also expressed in several tissues such as kidney, brain and liver. The primary structure of ASAH2/neutral ceramidase is highly conserved from bacteria to humans, however, there is a clear difference in the molecular architecture. The murine ASAH2 possesses 'amucin box', a Ser/Thr/Pro-rich domain glycosylated with O-glycans which is necessary to retain the enzyme on the plasma membrane as a type II integral protein. The major physiological function of ASAH2/neutral ceramidase is the metabolism of dietary sphingolipids, and thus plays a role in the generation of messenger molecules such as sphingosine and sphingosine 1-phosphate.

References

Tani M, et al. (2000) Molecular cloning of the full-length cDNA encoding mouse neutral ceramidase. A novel but highly conserved gene family of neutral/alkaline ceramidases. J Biol Chem. 275(15): 11229-34.Franzen R, et al. (2002) Nitric oxide induces neutral ceramidase degradation by the ubiquitin/proteasome complex in renal mesangial cell cultures. FEBS Lett. 532(3): 441-4.Kono M, et al. (2006) Neutral ceramidase encoded by the Asah2 gene is essential for the intestinal degradation of sphingolipids. J Biol Chem. 281(11): 7324-31.

NCBI and Uniprot Product Information

• NCBI GI #: 9055168
• NCBI GeneID: 54447
• NCBI Accession #: NP_061300.1
• NCBI GenBank Nucleotide #: NM_018830.1
• UniProt Accession #: Q9JHE3; Q3UWP9; Q8BNP0; Q8BQN7; Q8R236
• Molecular Weight: 83,509 Da
• NCBI Official Full Name: neutral ceramidase
• NCBI Official Synonym Full Names: N-acylsphingosine amidohydrolase 2
• NCBI Official Symbol: Asah2
• NCBI Official Synonym Symbols: AI585898
• NCBI Protein Information: neutral ceramidase; NCDase; N-CDase; acylsphingosine deacylase 2; neutral/alkaline ceramidase
• UniProt Protein Name: Neutral ceramidase
• Protein Family: Neutral ceramidase
• UniProt Gene Name: Asah2
• UniProt Synonym Gene Names: N-CDase; NCDase
• UniProt Entry Name: ASAH2_MOUSE