Human Periaxin ELISA Kit | PRX elisa kit

Human Periaxin ELISA Kit

Cat. Num. AAA763515

• Reactivity: Human
• Synonyms: Periaxin; Human Periaxin ELISA Kit; PRX (Periaxin); PRX elisa kit

• Reactivity: Human
• Sequence Length: 1383

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for PRX elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human Periaxin antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human Periaxin antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Periaxin amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Human Periaxin can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 18677712
• NCBI GeneID: 78960
• NCBI Accession #: NP_076466.2
• NCBI GenBank Nucleotide #: NM_023976.2
• Molecular Weight: 15,968 Da
• NCBI Official Full Name: periaxin
• NCBI Official Synonym Full Names: periaxin
• NCBI Official Symbol: Prx
• NCBI Protein Information: periaxin
• UniProt Protein Name: Periaxin
• Protein Family: Periaxin
• UniProt Gene Name: Prx
• UniProt Entry Name: PRAX_RAT

NCBI Description

may play a role in axonal ensheathment [RGD, Feb 2006]

Uniprot Description

PRX: Seems to be required for maintenance of peripheral nerve myelin sheath. May have a role in axon-glial interactions, possibly by interacting with the cytoplasmic domains of integral membrane proteins such as myelin-associated glycoprotein in the periaxonal regions of the Schwann cell plasma membrane. May have a role in the early phases of myelin deposition. Defects in PRX are a cause of Dejerine-Sottas syndrome (DSS); also known as Dejerine-Sottas neuropathy (DSN) or hereditary motor and sensory neuropathy III (HMSN3). DSS is a severe degenerating neuropathy of the demyelinating Charcot-Marie- Tooth disease category, with onset by age 2 years. DSS is characterized by motor and sensory neuropathy with very slow nerve conduction velocities, increased cerebrospinal fluid protein concentrations, hypertrophic nerve changes, delayed age of walking as well as areflexia. There are both autosomal dominant and autosomal recessive forms of Dejerine-Sottas syndrome. Belongs to the periaxin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell surface

Cellular Component: cell junction; cytoplasm; cytosol; myelin sheath; nucleus; plasma membrane

Molecular Function: protein binding

Biological Process: axon ensheathment; myelin formation in the peripheral nervous system; myelin maintenance in the peripheral nervous system; nerve development; regulation of RNA splicing; sensory perception of pain; transmission of nerve impulse