ARSG recombinant protein

ARSG, 17-525aa, Human, His tag, E Coli (Denatured)

Cat. Num. AAA205152

• Applications: SDS-Page
• Purity: > 85% by SDS-PAGE
• Synonyms: ARSG; 17-525aa; Human; His tag; E Coli (Denatured); Arylsulfatase G; ARSG recombinant protein

• Host: E Coli
• Purity/Purification: > 85% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.4M urea, 10% glycerol
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSGFLYPLV DFCISGKTRG QKPNFVIILA DDMGWGDLGA NWAETKDTAN LDKMASEGMR FVDFHAAAST CSPSRASLLT GRLGLRNGVT RNFAVTSVGG LPLNETTLAE VLQQAGYVTG IIGKWHLGHH GSYHPNFRGF DYYFGIPYSH DMGCTDTPGY NHPPCPACPQ GDGPSRNLQR DCYTDVALPL YENLNIVEQP VNLSSLAQKY AEKATQFIQR ASTSGRPFLL YVALAHMHVP LPVTQLPAAP RGRSLYGAGL WEMDSLVGQI KDKVDHTVKE NTFLWFTGDN GPWAQKCELA GSVGPFTGFW QTRQGGSPAK QTTWEGGHRV PALAYWPGRV PVNVTSTALL SVLDIFPTVV ALAQASLPQG RRFDGVDVSE VLFGRSQPGH RVLFHPNSGA AGEFGALQTV RLERYKAFYI TGGARACDGS TGPELQHKFP LIFNLEDDTA EAVPLERGGA EYQAVLPEVR KVLADVLQDI ANDNISSADY TQDPSVTPCC NPYQIACRCQ AA
• Sequence Length: 525

• Applicable Applications for ARSG recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for ARSG recombinant protein

ARSG belongs to the sulfatase enzyme family. Sulfatases hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This protein displays arylsulfatase activity at acidic pH, as is typical of lysosomal sulfatases, and has been shown to localize in the lysosomes. Alternatively spliced transcript variants have been found for this gene. Recombinant human ARSG protein, fused to His-tag at N-terminus, was expressed in E Coli.

Product Categories/Family for ARSG recombinant protein

Enzymes & Proteases

References

Frese MA, Schulz S, et al. (2008). J Biol Chem. 283 (17):11388-95.; Hong KW, Go MJ, et al. (2010). J Hum Hypertens. 24 (6):367-72.

NCBI and Uniprot Product Information

• NCBI GI #: 45430057
• NCBI GeneID: 22901
• NCBI Accession #: NP_055775
• NCBI GenBank Nucleotide #: NM_014960.4
• UniProt Accession #: Q96EG1; Q6UXF2; Q9Y2K4
• Molecular Weight: 57kDa (532aa)
• NCBI Official Full Name: arylsulfatase G isoform 1
• NCBI Official Synonym Full Names: arylsulfatase G
• NCBI Official Symbol: ARSG
• NCBI Protein Information: arylsulfatase G
• UniProt Protein Name: Arylsulfatase G
• Protein Family: Arylsulfatase
• UniProt Gene Name: ARSG
• UniProt Synonym Gene Names: KIAA1001; ASG

NCBI Description

The protein encoded by this gene belongs to the sulfatase enzyme family. Sulfatases hydrolyze sulfate esters from sulfated steroids, carbohydrates, proteoglycans, and glycolipids. They are involved in hormone biosynthesis, modulation of cell signaling, and degradation of macromolecules. This protein displays arylsulfatase activity at acidic pH, as is typical of lysosomal sulfatases, and has been shown to localize in the lysosomes. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Jun 2012]

Uniprot Description

Displays arylsulfatase activity at acidic pH with pseudosubstrates, such as p-nitrocatechol sulfate and also, but with lower activity, p-nitrophenyl sulfate and 4-methylumbelliferyl sulfate.