Human Aromatase ELISA Kit | ARO elisa kit

Human Aromatase ELISA Kit

Cat. Num. AAA763688

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Reactivity: Human
• Synonyms: Aromatase; Human Aromatase ELISA Kit; ARO/CYP19A1/ARO1/CPV1/CYAR/CYP19/CYPXIX/P-450AROM/estrogen synthetase/Cytochrome P-450AROM/Aromatase/CYPXIX; ARO elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of ARO . No significant cross-reactivity or interference between ARO and analogues was observed.
• Sequence Length: 503

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ARO were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ARO were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

NCBI and Uniprot Product Information

• NCBI GI #: 13904860
• NCBI GeneID: 1588
• NCBI Accession #: NP_112503.1
• NCBI GenBank Nucleotide #: NM_031226.2
• Molecular Weight: 24,518 Da
• NCBI Official Full Name: aromatase
• NCBI Official Synonym Full Names: cytochrome P450 family 19 subfamily A member 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: aromatase
• UniProt Protein Name: Aromatase
• Protein Family: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.14.14.14

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum; endoplasmic reticulum membrane; membrane

Molecular Function: electron carrier activity; heme binding; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; oxygen binding

Biological Process: androgen metabolic process; estrogen biosynthetic process; negative regulation of chronic inflammatory response; steroid biosynthetic process; steroid metabolic process; sterol metabolic process; xenobiotic metabolic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome