Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 ELISA Kit | CYP19A1 elisa kit

Human Cytochrome P450, Family 19, Subfamily A, Polypeptide 1 ELISA Kit

Cat. Num. AAA753313

• Gene Names: CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• Reactivity: Human
• Synonyms: Cytochrome P450; Family 19; Subfamily A; Polypeptide 1; Human Cytochrome P450; Polypeptide 1 ELISA Kit; CYP19A1 elisa kit

• Reactivity: Human

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 5.0-100ng/mL
• Sensitivity: 1.0ng/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP19A1 elisa kit

Principle of the assay: CYP19A1 ELISA kit applies the quantitative sandwich enzyme immunoassay technique. The microtiter plate has been pre-coated with a monoclonal antibody specific for CYP19A1. Standards or samples are then added to the microtiter plate wells and CYP19A1 if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of CYP19A1 present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for CYP19A1 are added to each well to “sandwich” the CYP19A1 immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, substrate solutions are added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period. Only those wells that contain CYP19A1 and enzyme-conjugated antibody will exhibit a change in color. The enzyme-substrate reaction is terminated by addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CYP19A1 concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 13904860
• NCBI GeneID: 1588
• NCBI Accession #: NP_112503.1
• NCBI GenBank Nucleotide #: NM_031226.2
• Molecular Weight: 24,518 Da
• NCBI Official Full Name: aromatase
• NCBI Official Synonym Full Names: cytochrome P450, family 19, subfamily A, polypeptide 1
• NCBI Official Symbol: CYP19A1
• NCBI Official Synonym Symbols: ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM
• NCBI Protein Information: aromatase; estrogen synthase; estrogen synthetase; cytochrome P-450AROM; cytochrome P450 19A1; microsomal monooxygenase; flavoprotein-linked monooxygenase; cytochrome P450, subfamily XIX (aromatization of androgens)
• UniProt Protein Name: Aromatase
• Protein Family: Aromatase
• UniProt Gene Name: CYP19A1
• UniProt Synonym Gene Names: ARO1; CYAR; CYP19
• UniProt Entry Name: CP19A_HUMAN

NCBI Description

This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum and catalyzes the last steps of estrogen biosynthesis. Mutations in this gene can result in either increased or decreased aromatase activity; the associated phenotypes suggest that estrogen functions both as a sex steroid hormone and in growth or differentiation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2014]

Uniprot Description

CYP19A1: Catalyzes the formation of aromatic C18 estrogens from C19 androgens. Belongs to the cytochrome P450 family.

Protein type: Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.14.14.14

Chromosomal Location of Human Ortholog: 15q21.1

Cellular Component: endoplasmic reticulum membrane; membrane; endoplasmic reticulum

Molecular Function: electron carrier activity; iron ion binding; oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, reduced flavin or flavoprotein as one donor, and incorporation of one atom of oxygen; heme binding; oxygen binding

Biological Process: steroid metabolic process; xenobiotic metabolic process; androgen metabolic process; estrogen biosynthetic process; sterol metabolic process; steroid biosynthetic process

Disease: Aromatase Deficiency; Aromatase Excess Syndrome