Typical Testing Data/Standard Curve (for reference only)
Human ARHGDIalpha (Rho GDP Dissociation Inhibitor Alpha) CLIA Kit | ARHGDIa clia kit
Human ARHGDIalpha (Rho GDP Dissociation Inhibitor Alpha) CLIA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to ARHGDIalpha. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ARHGDIalpha and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain ARHGDIalpha, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of ARHGDIalpha. You can calculate the concentration of ARHGDIalpha in the samples by comparing the RLU value of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that plays a key role in the regulation of signaling through Rho GTPases. The encoded protein inhibits the disassociation of Rho family members from GDP (guanine diphosphate), thereby maintaining these factors in an inactive state. Activity of this protein is important in a variety of cellular processes, and expression of this gene may be altered in tumors. Mutations in this gene have been found in individuals with nephrotic syndrome, type 8. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
Uniprot Description
RhoGDI alpha: Regulates the GDP/GTP exchange reaction of the Rho proteins by inhibiting the dissociation of GDP from them, and the subsequent binding of GTP to them. Monomer. Forms a heterodimer with RAC1. Interacts with FER. Belongs to the Rho GDI family.
Protein type: Cell adhesion; G protein regulator, misc.; Motility/polarity/chemotaxis; Apoptosis; Cell development/differentiation
Chromosomal Location of Human Ortholog: 17q25.3
Cellular Component: cytoskeleton; cytosol
Molecular Function: protein binding; Rho GDP-dissociation inhibitor activity; GTPase activator activity
Biological Process: regulation of small GTPase mediated signal transduction; nerve growth factor receptor signaling pathway; regulation of catalytic activity; small GTPase mediated signal transduction; positive regulation of axonogenesis; negative regulation of axonogenesis; negative regulation of cell adhesion; regulation of axonogenesis; cell motility; Rho protein signal transduction; negative regulation of apoptosis; positive regulation of GTPase activity
Disease: Nephrotic Syndrome, Type 8
Research Articles on ARHGDIa
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Product Notes
The Human ARHGDIa arhgdia (Catalog #AAA2531361) is a CLIA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2531361 CLIA Kit recognizes Human ARHGDIa. It is sometimes possible for the material contained within the vial of "ARHGDIalpha (Rho GDP Dissociation Inhibitor Alpha), CLIA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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