Arginase (ARG) Recombinant Protein | ARG recombinant protein

Recombinant Arginase (ARG)

Cat. Num. AAA2009782

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Arginase (ARG); Recombinant Arginase (ARG); ARG recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MSSKPKPIEI IGAPFSKGQP RGGVEKGPAA LRKAGLVEKL KETEYNVRDH GDLAFVDVPN DSPFQIVKNP RSVGKANEQL AAVVAETQKN GTISVVLGGD HSMAIGSISG HARVHPDLCV IWVDAHTDIN TPLTTSSGNL HGQPVAFLLK ELKGKFPDVP GFSWVTPCIS AKDIVYIGLR DVDPGEHYII KTLGIKYFSM TEVDKLGIGK VMEETFSYLL GRKKRPIHLS FDVDGLDPVF TPATGTPVVG GLSYREGLYI TEEIYKTGLL SGLDIMEVNP TLGKTPEEVT RTVNTAVALT LSCFGTKREG NHKPETDYLK PPK
• Sequence Length: 323

• Applicable Applications for ARG recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Lys323 (Accession # P07824) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 166091458
• NCBI GeneID: 29221
• NCBI Accession #: NP_058830.2
• NCBI GenBank Nucleotide #: NM_017134.3
• UniProt Accession #: P07824; Q5BK93
• Molecular Weight: 36.5kDa
• NCBI Official Full Name: arginase-1
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: Arg1
• NCBI Protein Information: arginase-1; arginase, liver; type I arginase; AI type I arginase; liver-type arginase
• UniProt Protein Name: Arginase-1
• UniProt Gene Name: Arg1
• UniProt Entry Name: ARGI1_RAT

NCBI Description

catalyzes the hydrolysis of arginine to ornithine and urea in arginine metabolism; regulates nitric oxide production [RGD, Feb 2006]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; Hydrolase; EC 3.5.3.1

Cellular Component: extracellular space; mitochondrial outer membrane; neuron projection; cell soma; cytoplasm; nucleus

Molecular Function: manganese ion binding; arginase activity

Biological Process: mammary gland involution; response to peptide hormone stimulus; maternal process involved in pregnancy; response to lipopolysaccharide; female pregnancy; response to vitamin A; response to selenium ion; response to vitamin E; response to methylmercury; response to wounding; response to axon injury; collagen biosynthetic process; aging; response to drug; response to herbicide; liver development; response to amino acid stimulus; response to manganese ion; response to cadmium ion; arginine metabolic process; response to steroid hormone stimulus; response to zinc ion; arginine catabolic process to ornithine; positive regulation of endothelial cell proliferation; urea cycle; lung development; response to amine stimulus