Human Arginase-1 ELISA Kit | ARG1 elisa kit

Human Arginase-1 (ARG1) ELISA Kit

Cat. Num. AAA284037

• Reactivity: Human
• Synonyms: Arginase-1; Human Arginase-1 (ARG1) ELISA Kit; ARG1; Arginase I; Liver Arginase; ARG1 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Human ARG1. No significant cross-reactivity or interference between Human ARG1 and analogues was observed.
• Sequence Length: 323

• Samples: Serum, plasma and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 1.56 ng/mL - 100 ng/mL
• Sensitivity: 0.52 ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay) Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays) Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. CV (%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for ARG1 elisa kit

Intended Uses: For the quantitative detection of Human Arginase-1 (ARG1) concentration in serum, plasma and other biological fluids.

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate ARG1 in Human serum, plasma. An antibody specific for ARG1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any ARG1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for ARG1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of ARG1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10947139
• NCBI GeneID: 383
• NCBI Accession #: NP_000036.2
• NCBI GenBank Nucleotide #: NM_000045.3
• UniProt Accession #: P05089; Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0
• Molecular Weight: 25,356 Da
• NCBI Official Full Name: arginase-1 isoform 2
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: ARG1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• Protein Family: Arginase
• UniProt Gene Name: ARG1

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]

Uniprot Description

ARG1: Homotrimer. By arginine or homoarginine. Belongs to the arginase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Amino Acid Metabolism - arginine and proline; EC 3.5.3.1; Hydrolase

Chromosomal Location of Human Ortholog: 6q23.2

Cellular Component: cell soma; cytoplasm; cytosol; extracellular region; extracellular space; mitochondrial outer membrane; neuron projection; nucleus

Molecular Function: arginase activity; manganese ion binding

Biological Process: aging; arginine catabolic process; arginine catabolic process to ornithine; collagen biosynthetic process; defense response to protozoan; liver development; lung development; mammary gland involution; maternal process involved in female pregnancy; negative regulation of activated T cell proliferation; negative regulation of T cell proliferation; neutrophil degranulation; positive regulation of endothelial cell proliferation; response to amine; response to amino acid; response to axon injury; response to cadmium ion; response to drug; response to herbicide; response to manganese ion; response to methylmercury; response to selenium ion; response to vitamin A; response to vitamin E; response to zinc ion; urea cycle

Disease: Argininemia