Human Arginase (Arg) ELISA Kit | Arg elisa kit

Human Arginase (Arg) ELISA Kit

Cat. Num. AAA454916

• Reactivity: Human
• Synonyms: Arginase (Arg); Human Arginase (Arg) ELISA Kit; ARG1; Arginase I; Liver Arginase; Arg elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Arg. No significant cross-reactivity or interference between Arg and analogues was observed.
• Sequence Length: 322

• Samples: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12-200ng/mL
• Sensitivity: < 1.35ng/mL
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Arg were tested 20 times on one plate, respectively. Intra-Assay: CV<10%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Arg were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100. Inter-Assay: CV<12%
• Preparation and Storage: For unopened kit, all reagents should be kept according to the labels on vials. The TMB Substrate, Wash Buffer, Stop Solution should be stored at 4 degree C. All others should be stored at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Arg elisa kit

Intended Uses: The kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of Arg in human serum, plasma, tissue homogenates, cell lysates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to Arg. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific to Arg. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Arg, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Arg in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 10947139
• NCBI GeneID: 383
• NCBI Accession #: NP_000036.2
• NCBI GenBank Nucleotide #: NM_000045.3
• UniProt Accession #: P05089; Q5JWT5; Q5JWT6; Q8TE72; Q9BS50; A6NEA0
• Molecular Weight: 25,356 Da
• NCBI Official Full Name: arginase-1 isoform 2
• NCBI Official Synonym Full Names: arginase 1
• NCBI Official Symbol: ARG1
• NCBI Protein Information: arginase-1
• UniProt Protein Name: Arginase-1
• UniProt Gene Name: ARG1

NCBI Description

Arginase catalyzes the hydrolysis of arginine to ornithine and urea. At least two isoforms of mammalian arginase exist (types I and II) which differ in their tissue distribution, subcellular localization, immunologic crossreactivity and physiologic function. The type I isoform encoded by this gene, is a cytosolic enzyme and expressed predominantly in the liver as a component of the urea cycle. Inherited deficiency of this enzyme results in argininemia, an autosomal recessive disorder characterized by hyperammonemia. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2011]