Porcine Amphiregulin ELISA Kit | AREG elisa kit

Porcine Amphiregulin ELISA Kit

Cat. Num. AAA743031

• Gene Names: AREG; AR; SDGF; AREGB; CRDGF
• Reactivity: Porcine
• Synonyms: Amphiregulin; Porcine Amphiregulin ELISA Kit; AREG elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of AREG. No significant cross-reactivity or interference between AREG and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AREG and all the analogues, therefore, cross reaction may still exist in some cases.

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Quantitative Competitive
• Sensitivity: 1.0 pg/mL
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for AREG elisa kit

Intended Uses: This AREG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine AREG. This ELISA kit for research use only!

Principle of the Assay: AREG ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-AREG antibody and an AREG-HRP conjugate. The assay sample and buffer are incubated together with AREG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AREG concentration since AREG from samples and AREG-HRP conjugate compete for the anti-AREG antibody binding site. Since the number of sites is limited, as more sites are occupied by AREG from the sample, fewer sites are left to bind AREG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AREG concentration in each sample is interpolated from this standard curve.

Product Categories/Family for AREG elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 179040
• NCBI GeneID: 374
• Molecular Weight: 27,895 Da
• NCBI Official Full Name: amphiregulin
• NCBI Official Synonym Full Names: amphiregulin
• NCBI Official Symbol: AREG
• NCBI Official Synonym Symbols: AR; SDGF; AREGB; CRDGF
• NCBI Protein Information: amphiregulin; amphiregulin B; schwannoma-derived growth factor; colorectum cell-derived growth factor
• UniProt Protein Name: Amphiregulin
• Protein Family: Amphiregulin
• UniProt Gene Name: AREG
• UniProt Synonym Gene Names: SDGF; AREGB; AR; CRDGF
• UniProt Entry Name: AREG_HUMAN

NCBI Description

The protein encoded by this gene is a member of the epidermal growth factor family. It is an autocrine growth factor as well as a mitogen for astrocytes, Schwann cells and fibroblasts. It is related to epidermal growth factor (EGF) and transforming growth factor alpha (TGF-alpha). The protein interacts with the EGF/TGF-alpha receptor to promote the growth of normal epithelial cells, and it inhibits the growth of certain aggressive carcinoma cell lines. It also functions in mammary gland, oocyte and bone tissue development. This gene is associated with a psoriasis-like skin phenotype, and is also associated with other pathological disorders, including various types of cancers and inflammatory conditions. [provided by RefSeq, Apr 2014]

Uniprot Description

AREG: Ligand of the EGF receptor/EGFR. Autocrine growth factor as well as a mitogen for a broad range of target cells including astrocytes, Schwann cells and fibroblasts. Belongs to the amphiregulin family.

Protein type: Membrane protein, integral

Chromosomal Location of Human Ortholog: 4q13.3

Cellular Component: extracellular space; cell surface; cytoplasm; integral to membrane; nucleus

Molecular Function: protein binding; growth factor activity; cytokine activity; epidermal growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; response to peptide hormone stimulus; response to cAMP; response to glucocorticoid stimulus; response to estradiol stimulus; glial cell proliferation; cell proliferation; G-protein coupled receptor protein signaling pathway; cell-cell signaling; response to hydrogen peroxide; positive regulation of cell proliferation; negative regulation of osteoblast differentiation; positive regulation of DNA replication; positive regulation of phosphorylation; neurite development