Typical Testing Data/Standard Curve (for reference only)
Human Aldose reductase ELISA Kit | AR elisa kit
Human AR (Aldose reductase) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AR. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for ARand Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AR.You can calculate the concentration of ARin the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. This member catalyzes the reduction of a number of aldehydes, including the aldehyde form of glucose, and is thereby implicated in the development of diabetic complications by catalyzing the reduction of glucose to sorbitol. Multiple pseudogenes have been identified for this gene. The nomenclature system used by the HUGO Gene Nomenclature Committee to define human aldo-keto reductase family members is known to differ from that used by the Mouse Genome Informatics database. [provided by RefSeq, Feb 2009]
Uniprot Description
AKR1B1: Catalyzes the NADPH-dependent reduction of a wide variety of carbonyl-containing compounds to their corresponding alcohols with a broad range of catalytic efficiencies. Belongs to the aldo/keto reductase family.
Protein type: Carbohydrate Metabolism - pyruvate; Carbohydrate Metabolism - fructose and mannose; Carbohydrate Metabolism - pentose and glucuronate interconversions; Carbohydrate Metabolism - galactose; Lipid Metabolism - glycerolipid; Oxidoreductase; EC 1.1.1.21
Chromosomal Location of Human Ortholog: 7q35
Cellular Component: cytoplasm; cytosol; extracellular space; mast cell granule; nucleoplasm; perinuclear region of cytoplasm
Molecular Function: aldehyde reductase activity; aldo-keto reductase activity; electron carrier activity; glyceraldehyde oxidoreductase activity
Biological Process: C21-steroid hormone biosynthetic process; carbohydrate metabolic process; fructose biosynthetic process; fructose metabolic process; maternal process involved in pregnancy; naphthalene metabolic process; norepinephrine metabolic process; positive regulation of JAK-STAT cascade; positive regulation of smooth muscle cell proliferation; response to stress; response to water deprivation; sorbitol biosynthetic process; steroid metabolic process; stress-activated protein kinase signaling pathway; tissue homeostasis
Research Articles on AR
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Product Notes
The Human AR akr1b1 (Catalog #AAA2533345) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2533345 ELISA Kit recognizes Human AR. It is sometimes possible for the material contained within the vial of "Aldose reductase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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