Aquaporin 9 Recombinant Protein | AQP9 recombinant protein

Recombinant Aquaporin 9 (AQP9)

Cat. Num. AAA2029653

• Gene Names: AQP9; SSC1; AQP-9; HsT17287
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: >95%
• Synonyms: Aquaporin 9; Recombinant Aquaporin 9 (AQP9); AQP9 recombinant protein

• Host: Host: E Coli; Source: Prokaryotic expression
• Purity/Purification: >95%
• Form/Format: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Concentration: 7.7 (varies by lot)
• Sequence: The target protein is fused with two N-terminal Tags, His-tag and T7-tag, its sequence is listed below.; MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- EKDRAKK NLVQRLALKS CLAKETLSEF LGTFIMIVLG CGSIAQAVLS REKAGGIITI NIGFATAVVM ALYATFGVSG GHINPAVSFA MCTFGRMEWF KFPFYVGAQL LGAFVGAATV FGIYYDGLMA FADGKLLITG ENGTAFIFAT YPKPFVSVPG AFVDQVVSTM FLLLIVFAIF DSRNLGVPRG LEPIVIGLLI IVISCSLGLN SGCAMNPARD LSPRLFTALA GWGFEVFTFG NNFWWIPVVG PMIGAVLGGLIYVLFIQMHH SNPDPEVKAE PAENN
• Sequence Length: 24

• Applicable Applications for AQP9 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Predicted Molecular Mass: 34.1kDa
• Accurate Molecular Mass (KD): 34kDa
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Expression System: Prokaryotic expression
• Tag: N-terminal His-Tag, T7 tag
• Organism Species: Mus musculus (Mouse)
• Fragment: Glu4~Asn285
• Usage: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. The loss of this protein is less than 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 6759671
• NCBI GeneID: 366
• Molecular Weight: 31,431 Da
• NCBI Official Full Name: aquaporin 9, partial
• NCBI Official Synonym Full Names: aquaporin 9
• NCBI Official Symbol: AQP9
• NCBI Official Synonym Symbols: SSC1; AQP-9; HsT17287
• NCBI Protein Information: aquaporin-9
• UniProt Protein Name: Aquaporin-9
• Protein Family: Aquaporin
• UniProt Gene Name: AQP9
• UniProt Synonym Gene Names: SSC1; AQP-9
• UniProt Entry Name: AQP9_HUMAN

NCBI Description

The aquaporins are a family of water-selective membrane channels. The protein encoded by this gene allows passage of a wide variety of noncharged solutes. It stimulates urea transport and osmotic water permeability; there are contradicting reports about its role in providing glycerol permeability. The encoded protein may also play a role in specialized leukocyte functions such as immunological response and bactericidal activity. [provided by RefSeq, Jul 2008]

Uniprot Description

AQP9: Forms a channel with a broad specificity. Mediates passage of a wide variety of non-charged solutes including carbamides, polyols, purines, and pyrimidines in a phloretin- and mercury-sensitive manner, whereas amino acids, cyclic sugars, Na(+), K(+), Cl(-), and deprotonated monocarboxylates are excluded. Also permeable to urea and glycerol. Belongs to the MIP/aquaporin (TC 1.A.8) family.

Protein type: Transporter, aquaporin family; Membrane protein, integral; Membrane protein, multi-pass; Channel, misc.; Transporter

Chromosomal Location of Human Ortholog: 15q

Cellular Component: intracellular membrane-bound organelle; integral to plasma membrane; basolateral plasma membrane; integral to membrane; plasma membrane

Molecular Function: purine transmembrane transporter activity; water transporter activity; pyrimidine transmembrane transporter activity; amine transmembrane transporter activity; urea transmembrane transporter activity; carboxylic acid transmembrane transporter activity; polyol transmembrane transporter activity; water channel activity; porin activity; glycerol channel activity

Biological Process: polyol transport; glycerol transport; cellular water homeostasis; metabolic process; water transport; amine transport; excretion; carboxylic acid transport; response to osmotic stress; purine transport; water homeostasis; response to mercury ion; response to organic substance; pyrimidine transport; transport; canalicular bile acid transport; immune response; transmembrane transport