Rabbit anti-Human HMMR Polyclonal Antibody | anti-HMMR antibody

HMMR, CT (HMMR, IHABP, RHAMM, Hyaluronan mediated motility receptor, Intracellular hyaluronic acid-binding protein, Receptor for hyaluronan-mediated motility, CD168) (MaxLight 650)

Cat. Num. AAA6307382

• Gene Names: HMMR; CD168; IHABP; RHAMM
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, Flow Cytometry, Functional Assay
• Purity: Purified by Protein A and Peptide Affinity Chromatography.
• Synonyms: HMMR; Polyclonal Antibody; CT (HMMR; IHABP; RHAMM; Hyaluronan mediated motility receptor; Intracellular hyaluronic acid-binding protein; Receptor for hyaluronan-mediated motility; CD168) (MaxLight 650); CD168; anti-HMMR antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HMMR.
• Purity/Purification: Purified by Protein A and Peptide Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with MaxLight650.

• Applicable Applications for anti-HMMR antibody: Immunohistochemistry (IHC) Paraffin, Western Blot (WB), Flow Cytometry (FC/FACS), FLISA
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 668-697 in the C-terminal region of human HMMR.
• Conjugate: MaxLight650
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: MaxLight650 conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot analysis in Ramos cell line lysates (35ug/lane) usingMBS646628.)

Western Blot (WB)

(Western Blot using MBS646628 (1:2000) Lane 1: K562 whole cell lysates Lane 2: LNCaP whole cell lysates Lane 3: HL-60 whole cell lysates. Lysates/proteins at 20ug/lane. S econdary: IgG (HRP) Goat Anti-Rabbit Predicted band size: 84kD Blocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)

(Immunohistochemistry analysis of formalin fixed and paraffin embedded human breast carcinoma using MBS646628 followed by peroxidase conjugation of the secondary antibody and DAB staining.)

Flow Cytometry (FC/FACS)

(Flow Cytometric analysis of Ramos cells using MBS646628 (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Product Categories/Family for anti-HMMR antibody

Antibodies; CD Markers

References

1. Huang, T.W., et al. Biomaterials 31(26):6701-6709 (2010). 2. Nagel, S., et al. Exp. Hematol. 38(1):38-45 (2010). 3. Gust, K.M., et al. Neoplasia 11(9):956-963 (2009). 4. Shigeishi, H., et al. Int. J. Oncol. 34(6):1565-1571(2009). 5. Luczynski, W., et al. Neoplasma 56(5):428-434 (2009).

NCBI and Uniprot Product Information

• NCBI GI #: 217416394
• NCBI GeneID: 3161
• NCBI Accession #: NP_036616.2
• NCBI GenBank Nucleotide #: NM_012484.2
• UniProt Accession #: O75330; Q32N02; Q92767; A8K3G2; B4E114; D3DQK9; D3DQL0; E9PCS0
• Molecular Weight: 74,495 Da
• NCBI Official Full Name: hyaluronan mediated motility receptor isoform b
• NCBI Official Synonym Full Names: hyaluronan-mediated motility receptor (RHAMM)
• NCBI Official Symbol: HMMR
• NCBI Official Synonym Symbols: CD168; IHABP; RHAMM
• NCBI Protein Information: hyaluronan mediated motility receptor; receptor for hyaluronan-mediated motility; intracellular hyaluronic acid-binding protein
• UniProt Protein Name: Hyaluronan mediated motility receptor
• Protein Family: Hyaluronan mediated motility receptor
• UniProt Gene Name: HMMR
• UniProt Synonym Gene Names: IHABP; RHAMM
• UniProt Entry Name: HMMR_HUMAN

NCBI Description

The protein encoded by this gene is involved in cell motility. It is expressed in breast tissue and together with other proteins, it forms a complex with BRCA1 and BRCA2, thus is potentially associated with higher risk of breast cancer. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene. [provided by RefSeq, Dec 2008]

Uniprot Description

HMMR: Involved in cell motility. When hyaluronan binds to HMMR, the phosphorylation of a number of proteins, including PTK2/FAK1 occurs. May also be involved in cellular transformation and metastasis formation, and in regulating extracellular- regulated kinase (ERK) activity. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Receptor, misc.

Chromosomal Location of Human Ortholog: 5q34

Cellular Component: cell surface; membrane; cytoplasm; plasma membrane

Molecular Function: hyaluronic acid binding

Biological Process: glycosaminoglycan metabolic process; carbohydrate metabolic process; pathogenesis; hyaluronan metabolic process; hyaluronan catabolic process

Disease: Breast Cancer