Typical Testing Data/Standard Curve (for reference only)
Rat AQP-2 ELISA Kit | AQP-2 elisa kit
Rat AQP-2 (Aquaporin 2) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay||This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to AQP-2. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for AQP-2 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain AQP-2, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of AQP-2. You can calculate the concentration of AQP-2 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a water channel protein located in the kidney collecting tubule. It belongs to the MIP/aquaporin family, some members of which are clustered together on chromosome 12q13. Mutations in this gene have been linked to autosomal dominant and recessive forms of nephrogenic diabetes insipidus. [provided by RefSeq, Oct 2008]
Uniprot Description
AQP2: Forms a water-specific channel that provides the plasma membranes of renal collecting duct with high permeability to water, thereby permitting water to move in the direction of an osmotic gradient. Expressed in renal collecting tubules. Belongs to the MIP/aquaporin (TC 1.A.8) family.
Protein type: Transporter; Transporter, aquaporin family; Membrane protein, multi-pass; Membrane protein, integral; Channel, misc.
Chromosomal Location of Human Ortholog: 12q12-q13
Cellular Component: recycling endosome; protein complex; membrane; rough endoplasmic reticulum; clathrin-coated vesicle; basolateral plasma membrane; integral to plasma membrane; lysosome; early endosome; apical plasma membrane; plasma membrane; trans-Golgi network
Molecular Function: water transporter activity; glycerol transmembrane transporter activity; water channel activity; actin binding; glycerol channel activity; PDZ domain binding
Biological Process: glycerol transport; actin filament depolymerization; cellular water homeostasis; response to glucagon stimulus; apoptosis; water transport; response to lithium ion; cellular response to water deprivation; response to lipopolysaccharide; female pregnancy; excretion; response to salt stress; response to starvation; hyperosmotic response; renal water transport; renal water homeostasis; response to calcium ion; positive regulation of calcium ion transport; transmembrane transport; aging
Disease: Diabetes Insipidus, Nephrogenic, Autosomal
Research Articles on AQP-2
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Product Notes
The Rat AQP-2 aqp2 (Catalog #AAA2515840) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2515840 ELISA Kit recognizes Rat AQP-2. It is sometimes possible for the material contained within the vial of "AQP-2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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