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Activity Data
(Figure 1. The chemotactic effect of EDN1 on THP1 cells.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 80ng/mL EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h. )
Endothelin 1 (EDN1) Active Protein | EDN1 active protein
Active Endothelin 1 (EDN1)
Gene Names
Edn1; Et1
Reactivity
Rat
Applications
Cell culture, Activity Assays
Purity
> 95%
Synonyms
Endothelin 1 (EDN1); Active Endothelin 1 (EDN1); ET1; PPET1; Preproendothelin-1; Big endothelin-1; EDN1 active protein
Host
E Coli
Reactivity
Rat
Purity/Purification
> 95%
Form/Format
Freeze-dried powder
Sequence Length
202
Applicable Applications for EDN1 active protein
Cell culture, Activity Assays
Tag
N-terminal His Tag
Buffer Formulation
20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
Fragment
Ser54~His202
Isoelectric Point
9.7
Activity Data
(Figure 1. The chemotactic effect of EDN1 on THP1 cells. (A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 80ng/mL EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h; (B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h. )
Activity Data
(Figure 1. The chemotactic effect of EDN1 on THP1 cells.
(A) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 with 80ng/mL EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h;
(B) THP-1 cells were seeded into the upper chambers and serum free RPMI 1640 without EDN1 was added in lower chamber, then cells in lower chamber were observed at low magnification (•100) after incubation for 3h. )
SDS-Page
SDS-Page
Testing Data
Testing Data
Testing Data
Testing Data
Product Categories/Family for EDN1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Predicted Molecular Weight: 18.5kDa
Accurate Molecular Weight: 19kDa
Accurate Molecular Weight: 19kDa
NCBI Official Full Name
endothelin-1 preproprotein
NCBI Official Synonym Full Names
endothelin 1
NCBI Official Symbol
Edn1
NCBI Official Synonym Symbols
Et1
NCBI Protein Information
endothelin-1
UniProt Protein Name
Endothelin-1
Protein Family
UniProt Gene Name
Edn1
UniProt Synonym Gene Names
ET-1; PPET1
NCBI Description
activates ERK1/2 mediated signaling; may play a role in regulation of cardiac contractile force [RGD, Feb 2006]
Uniprot Description
Endothelins are endothelium-derived vasoconstrictor peptides.
Research Articles on EDN1
Similar Products
Product Notes
The EDN1 edn1 (Catalog #AAA2097275) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The Active Endothelin 1 (EDN1) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Endothelin 1 (EDN1) can be used in a range of immunoassay formats including, but not limited to, Cell culture, Activity Assays. Researchers should empirically determine the suitability of the EDN1 edn1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Endothelin 1 (EDN1), Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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