APOC1 recombinant protein

APOC1, 27-83aa, Human, His tag, E Coli

Cat. Num. AAA205602

• Gene Names: APOC1; Apo-CI; ApoC-I; apo-CIB; apoC-IB
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: APOC1; 27-83aa; Human; His tag; E Coli; Apolipoprotein C-I precursor; APOC1B; Apo-CIB; ApoC-IB; Apolipoprotein C-I; Apolipoprotein C1; APOC1 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol.
• Concentration: 0.25 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSTPDVSSA LDKLKEFGNT LEDKARELIS RIKQSELSAK MREWFSETFQ KVKEKLKIDS
• Sequence Length: 83

• Applicable Applications for APOC1 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for APOC1 recombinant protein

APOC1 is a member of the apolipoprotein C family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. It is mapped to chromosome 19, where it resides within an apolipoprotein gene cluster. Alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. APOC1 has a length of 57 amino acids normally found in plasma and responsible for the activation of esterified lechitin cholesterol with an important role in the exchange of esterified cholesterol between lipoproteins and in removal of cholesterol from tissues. Its main function is inhibition of CETP, probably by altering the electric charge of HDL molecules. Recombinant human APOC1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for APOC1 recombinant protein

Metabolism

References

Gautier T, Masson D, et al. (2000). J Biol Chem. 1;275 (48):37504-9.; Kamino K, Yoshiiwa A, et al. (1996). Gerontology. Suppl 1:12-9.;

NCBI and Uniprot Product Information

• NCBI GI #: 4502157
• NCBI GeneID: 341
• NCBI Accession #: NP_001636
• NCBI GenBank Nucleotide #: NM_001645.4
• UniProt Accession #: P02654; Q6IB97; B2R526
• Molecular Weight: 9 kDa (80aa) confirmed by MALDI-TOF
• NCBI Official Full Name: apolipoprotein C-I
• NCBI Official Synonym Full Names: apolipoprotein C1
• NCBI Official Symbol: APOC1
• NCBI Official Synonym Symbols: Apo-CI; ApoC-I; apo-CIB; apoC-IB
• NCBI Protein Information: apolipoprotein C-I
• UniProt Protein Name: Apolipoprotein C-I
• Protein Family: Apolipoprotein
• UniProt Gene Name: APOC1
• UniProt Synonym Gene Names: Apo-CI; ApoC-I

NCBI Description

This gene encodes a member of the apolipoprotein C1 family. This gene is expressed primarily in the liver, and it is activated when monocytes differentiate into macrophages. The encoded protein plays a central role in high density lipoprotein (HDL) and very low density lipoprotein (VLDL) metabolism. This protein has also been shown to inhibit cholesteryl ester transfer protein in plasma. A pseudogene of this gene is located 4 kb downstream in the same orientation, on the same chromosome. This gene is mapped to chromosome 19, where it resides within a apolipoprotein gene cluster. Alternative splicing and the use of alternative promoters results in multiple transcript variants. [provided by RefSeq, Sep 2016]

Uniprot Description

Inhibitor of lipoprotein binding to the low density lipoprotein (LDL) receptor, LDL receptor-related protein, and very low density lipoprotein (VLDL) receptor. Associates with high density lipoproteins (HDL) and the triacylglycerol-rich lipoproteins in the plasma and makes up about 10% of the protein of the VLDL and 2% of that of HDL. Appears to interfere directly with fatty acid uptake and is also the major plasma inhibitor of cholesteryl ester transfer protein (CETP). Binds free fatty acids and reduces their intracellular esterification. Modulates the interaction of APOE with beta-migrating VLDL and inhibits binding of beta-VLDL to the LDL receptor-related protein.