JAM-2/JAM-B Active Protein | JAM2 active protein

JAM-2/JAM-B Protein, Rat, Recombinant (His Tag)

Cat. Num. AAA8123452

• Purity: >90% as determined by SDS-PAGE
• Synonyms: JAM-2/JAM-B; JAM-2/JAM-B Protein; Rat; Recombinant (His Tag); Rat JAM-2/JAM-B Protein (His Tag); junctional adhesion molecule 2; JAM2 active protein

• Host: HEK293 Cells
• Purity/Purification: >90% as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
• Sequence: Met1-Asn236

• Species: Rat
• Activity: Measured by the ability of the immobilized protein to support the adhesion of Jurkat human acute T cell leukemia cells. When cells (8 x 104 cells/well) are added to rat JAM2-His coated plates (5ug/mL, 100uL/well), >30% will adhere after 60 minutes at 37 degree C.
• Endotoxin: <1.0EU per ug of the protein as determined by the LAL method
• Predicted N Terminal: Phe 29
• Tag: C-His
• Protein Construction: A DNA sequence encoding the rat JAM2 (Q3MHC0)(Met1-Asn236) was expressed, fused with a polyhistidine tag at the C-terminus.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -20 degree C to -80 degree C. Store it under sterile conditions at -20 degree C to -80 degree C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.; In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid.; They are shipped out with blue ice.

SDS-Page

Related Product Information for JAM2 active protein

Background: Junctional adhesion molecule B (JAM-B), also known as Junctional adhesion molecule 2 (JAM2), Vascular endothelial junction-associated molecule (VE-JAM), and CD322, is a single-pass type I membrane protein that belongs to the immunoglobulin superfamily. It is prominently expressed on high endothelial venules. expression to be restricted to the high endothelial venule of tonsil and lymph nodes. The localization to the endothelium of arterioles in and around inflammatory and tumor foci. JAM-B can function as an adhesive ligand for the T cell line J45 and can interact with GM-CSF/IL-4-derived peripheral blood dendritic cells, circulating CD56(+) NK cells, circulating CD56(+)CD3(+) NK/T cells, and circulating CD56(+)CD3(+)CD8(+) cytolytic T cells. JAM-2 is expressed on high endothelial venules (HEVs) in human tonsil and on a subset of human leukocytes, suggesting that JAM-2 plays a central role in the regulation of transendothelial migration. It binds to very late activation antigen (VLA)-4, a leucocyte integrin that contributes to rolling and firm adhesion of lymphocytes to endothelial cells through binding to vascular cell adhesion molecule (VCAM)-1. JAM-B appears to contribute to leukocyte extravasation by facilitating not only transmigration but also rolling and adhesion. JAM-B acts as an adhesive ligand for interacting with a variety of immune cell types and may play a role in lymphocyte homing to secondary lymphoid organs.

References

Johnson-Lger CA, et al. (2002) Junctional adhesion molecule-2 (JAM-2) promotes lymphocyte transendothelial migration. Blood. 2100(7): 2479-86.Liang TW, et al. (2002) Vascular endothelial-junctional adhesion molecule (VE-JAM)/JAM 2 interacts with T, NK, and dendritic cells through JAM 3. J Immunol. 168(4): 1618-26.Ludwig RJ, et al. (2009) Junctional adhesion molecule (JAM)-B supports lymphocyte rolling and adhesion through interaction with alpha4beta1 integrin. Immunology. 128(2): 196-205.

NCBI and Uniprot Product Information

• NCBI GI #: 77404265
• NCBI GeneID: 619374
• NCBI Accession #: NP_001029176.1
• NCBI GenBank Nucleotide #: NM_001034004.1
• UniProt Accession #: Q3MHC0
• Molecular Weight: 33,058 Da
• NCBI Official Full Name: junctional adhesion molecule B
• NCBI Official Synonym Full Names: junctional adhesion molecule 2
• NCBI Official Symbol: Jam2
• NCBI Protein Information: junctional adhesion molecule B
• UniProt Protein Name: Junctional adhesion molecule 2
• Protein Family: Junctional adhesion molecule
• UniProt Gene Name: Jam2