Rabbit CA4 Polyclonal Antibody | anti-CA4 antibody

CA4 Polyclonal Antibody

Cat. Num. AAA3007654

• Gene Names: CA4; CAIV; Car4; RP17
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: CA4; Polyclonal Antibody; CA4 Polyclonal Antibody; Carbonic anhydrase 4; Carbonate dehydratase IV; Carbonic anhydrase IV; CA-IV; anti-CA4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: CA4 polyclonal antibody detects endogenous levels of CA4 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 312

• Applicable Applications for anti-CA4 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 120-170 of Human CA4.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of CA4 polyclonal antibody at 1:500 dilutionLane1:HEK293T whole cell lysate (40ug) Lane2:HepG2 whole cell lysate (40ug) Lane3:L02 whole cell lysate (40ug) Lane4:AML-12 whole cell lysate (40ug) Lane5:PC12 whole cell lysate (40ug))

Related Product Information for anti-CA4 antibody

Carbonic anhydrase IV (CA IV) is glycosylphosphotidylinositol-anchored to the outer surface of the plasma membrane where it catalyzes hydration-dehydration of CO2/HCO3 -. CA IV is present on the plasma face of microcapillaries and in the choriocapillaris of the human eye. CA IV facilitates renal acidification in the kidney and is responsible for the regulation of interstitial pH (pHo) transients in brain. Impairment in targetting leads to disruption of HCO3 - secretion and associates with malfunction in cystic fibrosis cells. Carbonic anhydrases are zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. Carbonic anhydrases show extensive diversity in tissue distribution and in their subcellular localization.

NCBI and Uniprot Product Information

• NCBI GI #: 115465
• NCBI GeneID: 762
• UniProt Accession #: P22748
• Molecular Weight: ~ 35kDa
• NCBI Official Full Name: Carbonic anhydrase 4
• NCBI Official Synonym Full Names: carbonic anhydrase 4
• NCBI Official Symbol: CA4
• NCBI Official Synonym Symbols: CAIV; Car4; RP17
• NCBI Protein Information: carbonic anhydrase 4
• UniProt Protein Name: Carbonic anhydrase 4
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA4
• UniProt Synonym Gene Names: CA-IV
• UniProt Entry Name: CAH4_HUMAN

NCBI Description

Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. This gene encodes a glycosylphosphatidyl-inositol-anchored membrane isozyme expressed on the luminal surfaces of pulmonary (and certain other) capillaries and proximal renal tubules. Its exact function is not known; however, it may have a role in inherited renal abnormalities of bicarbonate transport. [provided by RefSeq, Jul 2008]

Uniprot Description

CA4: Reversible hydration of carbon dioxide. May stimulate the sodium/bicarbonate transporter activity of SLC4A4 that acts in pH homeostasis. It is essential for acid overload removal from the retina and retina epithelium, and acid release in the choriocapillaris in the choroid. Defects in CA4 are the cause of retinitis pigmentosa type 17 (RP17). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP17 inheritance is autosomal dominant. Defective acid overload removal from retina and retinal epithelium, due to mutant CA4, is responsible for photoreceptor degeneration, indicating that impaired pH homeostasis is the most likely cause underlying the RP17 phenotype. Belongs to the alpha-carbonic anhydrase family.

Protein type: Lyase; Energy Metabolism - nitrogen; Membrane protein, GPI anchor; EC 4.2.1.1

Chromosomal Location of Human Ortholog: 17q23

Cellular Component: Golgi apparatus; cell surface; sarcoplasmic reticulum; rough endoplasmic reticulum; basolateral plasma membrane; brush border membrane; secretory granule membrane; ER-Golgi intermediate compartment; trans-Golgi network; anchored to external side of plasma membrane; membrane; perinuclear region of cytoplasm; apical plasma membrane; plasma membrane; sarcolemma

Molecular Function: carbonate dehydratase activity; protein binding; zinc ion binding

Biological Process: response to drug; response to steroid hormone stimulus; bicarbonate transport; organ development; one-carbon compound metabolic process

Disease: Retinitis Pigmentosa 17