SDS-Page
POLR3H recombinant protein
POLR3H, 1-204aa, Human, His tag, E Coli
Gene Names
POLR3H; RPC8; RPC22.9
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
POLR3H; 1-204aa; Human; His tag; E Coli; DNA-directed RNA polymerase III subunit RPC8; RPC22.9; RPC8; POLR3H recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
Concentration
0.5 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN
Sequence Length
204
Applicable Applications for POLR3H recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for POLR3H recombinant protein
POLR3H, also as known as A1665 and RPC8, belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. POLR3H is DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. It plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. POLR3H acts as nuclear and cytosolic DNA sensor involved in innate immune response. Recombinant human POLR3H protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for POLR3H recombinant protein
References
Ablasser A., et al. (2009) Nat. Immunol. 10:1065-1072; Hu P., et al. (2002) Mol. Cell. Biol. 22:8044-8055
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
25.3kDa (227aa) confirmed by MALDI-TOF (Molecular size on SDS-PAGE will appear higher)
NCBI Official Full Name
DNA-directed RNA polymerase III subunit RPC8 isoform a
NCBI Official Synonym Full Names
RNA polymerase III subunit H
NCBI Official Symbol
POLR3H
NCBI Official Synonym Symbols
RPC8; RPC22.9
NCBI Protein Information
DNA-directed RNA polymerase III subunit RPC8
UniProt Protein Name
DNA-directed RNA polymerase III subunit RPC8
Protein Family
UniProt Gene Name
POLR3H
UniProt Synonym Gene Names
KIAA1665; RPC8; RNA polymerase III subunit C8; RPC22.9
Uniprot Description
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Specific peripheric component of RNA polymerase III which synthesizes small RNAs, such as 5S rRNA and tRNAs. Plays a key role in sensing and limiting infection by intracellular bacteria and DNA viruses. Acts as nuclear and cytosolic DNA sensor involved in innate immune response. Can sense non-self dsDNA that serves as template for transcription into dsRNA. The non-self RNA polymerase III transcripts, such as Epstein-Barr virus-encoded RNAs (EBERs) induce type I interferon and NF- Kappa-B through the RIG-I pathway ().
Research Articles on POLR3H
Similar Products
Product Notes
The POLR3H polr3h (Catalog #AAA204467) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's POLR3H can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the POLR3H polr3h for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMFVLVEM VDTVRIPPWQ FERKLNDSIA EELNKKLANK VVYNVGLCIC LFDITKLEDA YVFPGDGASH TKVHFRCVVF HPFLDEILIG KIKGCSPEGV HVSLGFFDDI LIPPESLQQP AKFDEAEQVW VWEYETEEGA HDLYMDTGEE IRFRVVDESF VDTSPTGPSS ADATTSSEEL PKKEAPYTLV GSISEPGLGL LSWWTSN. It is sometimes possible for the material contained within the vial of "POLR3H, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
