Typical Testing Data/Standard Curve (for reference only)
(Rat SAP/PTX2 PicoKine ELISA Kit standard curve )
Rat SAP/PTX2 ELISA Kit | APCS elisa kit
Rat SAP/PTX2 PicoKine ELISA Kit
Gene Names
Apcs; Sap
Reactivity
Rat
Synonyms
SAP/PTX2; Rat SAP/PTX2 PicoKine ELISA Kit; Serum amyloid P-component; amyloid P component; serum; 9.5S alpha-1-glycoprotein; APCS; SAMP_HUMAN; SAP; Serum amyloid P-component(1-203); APCS elisa kit
Reactivity
Rat
Specificity
Natural and recombinant rat SAP
Sequence Length
228
Samples
Cell culture supernates, serum and plasma (heparin, EDTA)
Assay Type
Sandwich
Detection Range
1.56ng/ml-100ng/ml
Sensitivity
< 50pg/ml
Intra-assay Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-assay Precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Preparation and Storage
Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Typical Testing Data/Standard Curve (for reference only)
(Rat SAP/PTX2 PicoKine ELISA Kit standard curve )
Typical Testing Data/Standard Curve (for reference only)
(Rat SAP/PTX2 PicoKine ELISA Kit standard curve )
Related Product Information for APCS elisa kit
Principle of the assay: Rat SAP ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for SAP has been precoated onto 96-well plates. Standards(NSO, Q21-S228) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for SAP is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the rat SAP amount of sample captured in plate.
Background: Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
Background: Amyloid P component, serum (SAP), also known as PTX2 or APCS, is the identical serum form of amyloid P component (AP), a 25kDa pentameric protein first identified as the pentagonal constituent of in vivo pathological deposits called "amyloid". It belongs to the pentraxins family, characterised by calcium dependent ligand binding and distinctive flattened beta-jellyroll structure similar to that of the legume lectins. This gene is mapped to 1q23.2. The binding of the encoded protein to proteins in the pathological amyloid cross-beta fold suggests its possible role as a chaperone. This protein is also thought to control the degradation of chromatin. It has been demonstrated that this protein binds to apoptotic cells at an early stage, which raises the possibility that it is involved in dealing with apoptotic cells in vivo.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
26,176 Da
NCBI Official Full Name
Serum amyloid P-component
NCBI Official Synonym Full Names
amyloid P component, serum
NCBI Official Symbol
Apcs
NCBI Official Synonym Symbols
Sap
NCBI Protein Information
serum amyloid P-component
UniProt Protein Name
Serum amyloid P-component
UniProt Gene Name
Apcs
UniProt Synonym Gene Names
Ptx2; Sap; SAP
UniProt Entry Name
SAMP_RAT
NCBI Description
a component of amyloid P [RGD, Feb 2006]
Research Articles on APCS
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Product Notes
The Rat APCS apcs (Catalog #AAA177292) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA177292 ELISA Kit recognizes Rat APCS. It is sometimes possible for the material contained within the vial of "SAP/PTX2, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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