Mouse Membrane primary amine oxidase ELISA Kit | Aoc3 elisa kit

Mouse Membrane primary amine oxidase ELISA Kit

Cat. Num. AAA764876

• Gene Names: AOC3; HPAO; SSAO; VAP1; VAP-1
• Reactivity: Mouse
• Synonyms: Membrane primary amine oxidase; Mouse Membrane primary amine oxidase ELISA Kit; Aoc3/AOC3/VAP-1/HPAO/SSAO/Copper amine oxidase/HPAOSSAO/Semicarbazide-sensitive amine oxidase/VAP1/membrane primary amine oxidase/Vascular adhesion protein 1; Aoc3 elisa kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aoc3. No significant cross-reactivity or interference between Aoc3 and analogues was observed.
• Sequence Length: 634

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 31.25-2000pg/ml
• Sensitivity: 18.75pg/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Aoc3 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 480306390
• NCBI GeneID: 8639
• NCBI Accession #: NP_001264660.1
• NCBI GenBank Nucleotide #: NM_001277731.1
• Molecular Weight: 24,533 Da
• NCBI Official Full Name: membrane primary amine oxidase isoform 2
• NCBI Official Synonym Full Names: amine oxidase, copper containing 3
• NCBI Official Symbol: AOC3
• NCBI Official Synonym Symbols: HPAO; SSAO; VAP1; VAP-1
• NCBI Protein Information: membrane primary amine oxidase
• UniProt Protein Name: Membrane primary amine oxidase
• Protein Family: Membrane primary amine oxidase
• UniProt Gene Name: AOC3
• UniProt Synonym Gene Names: VAP1; SSAO; VAP-1
• UniProt Entry Name: AOC3_HUMAN

NCBI Description

This gene encodes a member of the semicarbazide-sensitive amine oxidase family. Copper amine oxidases catalyze the oxidative conversion of amines to aldehydes in the presence of copper and quinone cofactor. The encoded protein is localized to the cell surface, has adhesive properties as well as monoamine oxidase activity, and may be involved in leukocyte trafficking. Alterations in levels of the encoded protein may be associated with many diseases, including diabetes mellitus. A pseudogene of this gene has been described and is located approximately 9-kb downstream on the same chromosome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2013]

Uniprot Description

AOC3: Cell adhesion protein that participates in lymphocyte recirculation by mediating the binding of lymphocytes to peripheral lymph node vascular endothelial cells in an L-selectin- independent fashion. Has a monoamine oxidase activity. May play a role in adipogenesis. Belongs to the copper/topaquinone oxidase family.

Protein type: Other Amino Acids Metabolism - beta-alanine; Amino Acid Metabolism - phenylalanine; EC 1.4.3.21; Oxidoreductase; Membrane protein, integral; Amino Acid Metabolism - tyrosine; Amino Acid Metabolism - glycine, serine and threonine; Cell adhesion

Chromosomal Location of Human Ortholog: 17q21

Cellular Component: cell surface; cytoplasm; early endosome; endoplasmic reticulum; Golgi apparatus; integral to membrane; microvillus; plasma membrane

Molecular Function: amine oxidase activity; calcium ion binding; copper ion binding; protein binding; protein heterodimerization activity; protein homodimerization activity; quinone binding

Biological Process: amine metabolic process; cell adhesion; inflammatory response; response to antibiotic; xenobiotic metabolic process