Human Annexin A3 ELISA Kit | ANXA3 elisa kit

Human Annexin A3 ELISA Kit

Cat. Num. AAA762597

• Gene Names: ANXA3; ANX3
• Reactivity: Human
• Synonyms: Annexin A3; Human Annexin A3 ELISA Kit; ANXA3/Annexin A3/Calcimedin 35-alpha/Lipocortin III/PAP-III/35-alpha calcimedin/annexin A3; 1; 2-cyclic-inositol-phosphate phosphodiesterase/Annexin III/Annexin-3; ANXA3 elisa kit

• Reactivity: Human
• Sequence Length: 323

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 0.313-20ng/ml
• Sensitivity: 0.188ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for ANXA3 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Human ANXA3antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Human ANXA3 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human ANXA3 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofHuman ANXA3can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 4826643
• NCBI GeneID: 306
• NCBI Accession #: NP_005130.1
• NCBI GenBank Nucleotide #: NM_005139.2
• Molecular Weight: 36,375 Da
• NCBI Official Full Name: annexin A3
• NCBI Official Synonym Full Names: annexin A3
• NCBI Official Symbol: ANXA3
• NCBI Official Synonym Symbols: ANX3
• NCBI Protein Information: annexin A3
• UniProt Protein Name: Annexin A3
• Protein Family: Annexin
• UniProt Gene Name: ANXA3
• UniProt Synonym Gene Names: ANX3; PAP-III
• UniProt Entry Name: ANXA3_HUMAN

NCBI Description

This gene encodes a member of the annexin family. Members of this calcium-dependent phospholipid-binding protein family play a role in the regulation of cellular growth and in signal transduction pathways. This protein functions in the inhibition of phopholipase A2 and cleavage of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. This protein may also play a role in anti-coagulation. [provided by RefSeq, Jul 2008]

Uniprot Description

ANXA3: a calcium/phospholipid-binding protein and inhibitor of phospholipase A2, Possesses anti-coagulant properties. Cleaves the cyclic bond of inositol 1,2-cyclic phosphate to form inositol 1-phosphate. Annexins are a family of structurally related proteins whose common property is calcium-dependent binding to phospholipids. There are at least ten different annexins in mammalian species. Annexins do not contain signal peptides, yet some annexins (A1, A2 and A5) appear to be secreted in a physiologically regulated fashion.

Protein type: Calcium-binding

Chromosomal Location of Human Ortholog: 4q21.21

Cellular Component: axon; cell soma; cytoplasm; dendrite; membrane; phagocytic vesicle membrane; plasma membrane; specific granule

Molecular Function: calcium ion binding; calcium-dependent phospholipid binding; calcium-dependent protein binding; phospholipase A2 inhibitor activity

Biological Process: defense response to bacterium; hippocampus development; negative regulation of catalytic activity; neutrophil degranulation; organ regeneration; phagocytosis; positive regulation of angiogenesis; positive regulation of DNA metabolic process; positive regulation of transcription factor activity; response to glucocorticoid stimulus