Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 Antibody | anti-ENPP1 antibody
Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 (ENPP1, PC1, Plasma Cell Membrane Glycoprotein)
Immunohistochemistry (Paraffin): 1:200 Requires HIER using sodium citrate buffer pH6.0 prior to staining.
ELISA (Peptide): 1:2000
Optimal dilutions to be determined by the researcher.
NCBI and Uniprot Product Information
NCBI Description
This gene is a member of the ecto-nucleotide pyrophosphatase/phosphodiesterase (ENPP) family. The encoded protein is a type II transmembrane glycoprotein comprising two identical disulfide-bonded subunits. This protein has broad specificity and cleaves a variety of substrates, including phosphodiester bonds of nucleotides and nucleotide sugars and pyrophosphate bonds of nucleotides and nucleotide sugars. This protein may function to hydrolyze nucleoside 5' triphosphates to their corresponding monophosphates and may also hydrolyze diadenosine polyphosphates. Mutations in this gene have been associated with 'idiopathic' infantile arterial calcification, ossification of the posterior longitudinal ligament of the spine (OPLL), and insulin resistance. [provided by RefSeq]
Uniprot Description
ENPP1: Involved primarily in ATP hydrolysis at the plasma membrane. Plays a role in regulating pyrophosphate levels, and functions in bone mineralization and soft tissue calcification. In vitro, has a broad specificity, hydrolyzing other nucleoside 5' triphosphates such as GTP, CTP, TTP and UTP to their corresponding monophosphates with release of pyrophosphate and diadenosine polyphosphates, and also 3',5'-cAMP to AMP. May also be involved in the regulation of the availability of nucleotide sugars in the endoplasmic reticulum and Golgi, and the regulation of purinergic signaling. Appears to modulate insulin sensitivity. Homodimer; disulfide-linked. Interacts with INSR. Expressed in plasma cells and also in a number of non-lymphoid tissues, including the distal convoluted tubule of the kidney, chondrocytes and epididymis. At low concentrations of ATP, a phosphorylated intermediate is formed which inhibits further hydrolysis. Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.
Protein type: Membrane protein, integral; Nucleotide Metabolism - purine; Cofactor and Vitamin Metabolism - riboflavin; Carbohydrate Metabolism - starch and sucrose; Cofactor and Vitamin Metabolism - nicotinate and nicotinamide; Cofactor and Vitamin Metabolism - pantothenate and CoA biosynthesis; EC 3.6.1.9; Motility/polarity/chemotaxis; Phosphodiesterase; EC 3.1.4.1; Phosphatase (non-protein)
Chromosomal Location of Human Ortholog: 6q22-q23
Cellular Component: extracellular space; cell surface; integral to plasma membrane; basolateral plasma membrane; lysosomal membrane; plasma membrane; integral to membrane
Molecular Function: phosphodiesterase I activity; protein binding; nucleotide diphosphatase activity; protein homodimerization activity; 3'-phosphoadenosine 5'-phosphosulfate binding; nucleic acid binding; zinc ion binding; calcium ion binding; nucleoside-triphosphate diphosphatase activity; insulin receptor binding; scavenger receptor activity; ATP binding; polysaccharide binding
Biological Process: receptor-mediated endocytosis; sequestering of triacylglycerol; generation of precursor metabolites and energy; vitamin metabolic process; nucleoside triphosphate catabolic process; negative regulation of insulin receptor signaling pathway; bone remodeling; negative regulation of fat cell differentiation; phosphate metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process; riboflavin metabolic process; negative regulation of glucose import; cellular phosphate ion homeostasis; cellular response to insulin stimulus; biomineral formation; negative regulation of ossification; immune response; negative regulation of protein amino acid autophosphorylation; negative regulation of cell growth; regulation of bone mineralization; water-soluble vitamin metabolic process; inorganic diphosphate transport; negative regulation of glycogen biosynthetic process
Disease: Obesity; Cole Disease; Arterial Calcification, Generalized, Of Infancy, 1; Hypophosphatemic Rickets, Autosomal Recessive, 2; Diabetes Mellitus, Noninsulin-dependent
Research Articles on ENPP1
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Product Notes
The ENPP1 enpp1 (Catalog #AAA630983) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Ectonucleotide Pyrophosphatase, Phosphodiesterase 1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry. (IHC). Western Blot: 1:1000 Immunohistochemistry (Paraffin): 1:200 Requires HIER using sodium citrate buffer pH6.0 prior to staining. ELISA (Peptide): 1:2000 Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the ENPP1 enpp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Ectonucleotide Pyrophosphatase, Phosphodiesterase 1, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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