Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARAF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.)
Rabbit anti-Human, Mouse A-Raf Polyclonal Antibody | anti-ARAF antibody
A-Raf Rabbit pab Antibody
Gene Names
ARAF; PKS2; A-RAF; ARAF1; RAFA1
Reactivity
Human, Mouse
Applications
Western Blot, Immunohistochemistry
Purity
Affinity purification
Synonyms
A-Raf; Polyclonal Antibody; A-Raf Rabbit pab Antibody; ARAF; A-Raf ; ARAF1; PKS2; RAFA1; A-RAF; PKS;; anti-ARAF antibody
Host
Rabbit
Reactivity
Human, Mouse
Clonality
Polyclonal
Isotype
IgG
Purity/Purification
Affinity purification
Form/Format
Liquid
Sequence Length
609
Applicable Applications for anti-ARAF antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Western Blot (WB): 1/500-1000
Immunocytochemistry (ICC): 1/50-100
Immunofluorescence (IF): 1/20-50
Immunocytochemistry (ICC): 1/50-100
Immunofluorescence (IF): 1/20-50
Immunogen
A synthetic -peptide of human A-Raf
Buffer
PBS with 0.1% Sodium Azide, 50% Glycerol
Preparation and Storage
Upon delivery aliquot and store at -20 degree C or -80 degree C.
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARAF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.)
Western Blot (WB)
(Western blot analysis of extracts of various cell lines, using ARAF antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 5s.)
Related Product Information for anti-ARAF antibody
Rabbit polyclonal to Human A-Raf
A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).
A-Raf, B-Raf and c-Raf (Raf-1) are the main effectors recruited by GTP-bound Ras to activate the MEK-MAP kinase pathway (1). Activation of c-Raf is the best understood and involves phosphorylation at multiple activating sites including Ser338, Tyr341, Thr491, Ser494, Ser497 and Ser499 (2). p21-activated protein kinase (PAK) has been shown to phosphorylate c-Raf at Ser338 and the Src family phosphorylates Tyr341 to induce c-Raf activity (3,4). Ser338 of c-Raf corresponds to similar sites in A-Raf (Ser299) and B-Raf (Ser445), although this site is constitutively phosphorylated in B-Raf (5). Inhibitory 14-3-3 binding sites on c-Raf (Ser259 and Ser621) can be phosphorylated by Akt and AMPK, respectively (6,7). While A-Raf, B-Raf and c-Raf are similar in sequence and function, differential regulation has been observed (8). Of particular interest, B-Raf contains three consensus Akt phosphorylation sites (Ser364, Ser428 and Thr439) and lacks a site equivalent to Tyr341 of c-Raf (8,9). The B-Raf mutation V600E results in elevated kinase activity and is commonly found in malignant melanoma (10). Six residues of c-Raf (Ser29, Ser43, Ser289, Ser296, Ser301 and Ser642) become hyperphosphorylated in a manner consistent with c-Raf inactivation. The hyperphosphorylation of these six sites is dependent on downstream MEK signaling and renders c-Raf unresponsive to subsequent activation events (11).
References
1. Avruch, J. et al. (1994) Trends Biochem. Sci. 19, 279-283. 2. Chong, H. et al. (2001) EMBO J. 20, 3716-3727. 3. King, A.J. et al. (1998) Nature 396, 180-183. 4. Fabian, J.R. et al. (1993) Mol. Cell Biol. 13, 7170-7179. 5. Mason, C.S. et al. (1999) EMBO J. 18, 2137-2148. 6. Zimmermann, S. and Moelling, K. (1999) Science 286, 1741-1744. 7. Sprenkle, A.B. et al. (1997) FEBS Lett. 403, 254-258. 8. Marais, R. et al. (1997) J. Biol. Chem. 272, 4378-4383. 9. Guan, K.L. et al. (2000) J. Biol. Chem. 275, 27354-27359. 10. Davies, H. et al. (2002) Nature 417, 949-954. 11. Dougherty, M.K. et al. (2005) Mol. Cell 17, 215-224.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
606
NCBI Official Full Name
serine/threonine-protein kinase A-Raf isoform 2
NCBI Official Synonym Full Names
v-raf murine sarcoma 3611 viral oncogene homolog
NCBI Official Symbol
ARAF
NCBI Official Synonym Symbols
PKS2; A-RAF; ARAF1; RAFA1
NCBI Protein Information
serine/threonine-protein kinase A-Raf; Oncogene ARAF1; proto-oncogene Pks; proto-oncogene A-Raf-1; Ras-binding protein DA-Raf; v-raf murine sarcoma 3611 viral oncogene homolog 1; A-Raf proto-oncogene serine/threonine-protein kinase
UniProt Protein Name
Serine/threonine-protein kinase A-Raf
Protein Family
UniProt Gene Name
ARAF
UniProt Synonym Gene Names
ARAF1; PKS; PKS2
UniProt Entry Name
ARAF_HUMAN
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Product Notes
The ARAF araf (Catalog #AAA125368) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The A-Raf Rabbit pab Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's A-Raf can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Western Blot (WB): 1/500-1000 Immunocytochemistry (ICC): 1/50-100 Immunofluorescence (IF): 1/20-50. Researchers should empirically determine the suitability of the ARAF araf for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "A-Raf, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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