PKC Isoform Antibody Kit | PKC antibody kit

PKC Isoform Antibody Sampler Kit

Cat. Num. AAA2550094

• Applications: Western Blot
• Synonyms: PKC Isoform; PKC Isoform Antibody Sampler Kit; PKC antibody kit

• Applicable Applications for PKC antibody kit: Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of NIH-3T3 cells with PKC alpha Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of COS7 cells treated with PMA (125ng/ml, 30mins) minus immunising peptide using PKC zeta Polyclonal Antibody at dilution of 1:2000.)

Western Blot (WB)

(Western Blot analysis of 293T cells using PKD1 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of Jurkat cells using PKC delta Polyclonal Antibody at dilution of 1:1000.)

Related Product Information for PKC antibody kit

Background: Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCu (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity.