Rabbit PRKCG Polyclonal Antibody | anti-PRKCG antibody

PRKCG Polyclonal Antibody

Cat. Num. AAA3007439

• Gene Names: PRKCG; PKCC; PKCG; SCA14; PKCI(3); PKCgamma; PKC-gamma
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Synonyms: PRKCG; Polyclonal Antibody; PRKCG Polyclonal Antibody; Protein kinase C gamma type; PKC-gamma; anti-PRKCG antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: PRKCG polyclonal antibody detects endogenous levels of PRKCG protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE)
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 697

• Applicable Applications for anti-PRKCG antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:1000
• Immunogen: Synthetic peptide, corresponding to amino acids 543-587 of Human PRKCG
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of PRKCG polyclonal antibody at 1:500 dilutionLane1:A549 whole cell lysate (40ug) Lane2:The Brain tissue lysate of Rat (40ug) Lane3:The Placenta tissue lysate of Mouse (40ug))

Related Product Information for anti-PRKCG antibody

Activation of protein kinase C (PKC) is one of the earliest events in a cascade that controls a variety of cellular responses, including secretion, gene expression, proliferation, and muscle contraction. PKC isoforms belong to three groups based on calcium dependency and activators. Classical PKCs are calcium-dependent via their C2 domains and are activated by phosphatidylserine (PS), diacylglycerol (DAG), and phorbol esters (TPA, PMA) through their cysteine-rich C1 domains. Both novel and atypical PKCs are calcium-independent, but only novel PKCs are activated by PS, DAG, and phorbol esters. Members of these three PKC groups contain a pseudo-substrate or autoinhibitory domain that binds to substrate-binding sites in the catalytic domain to prevent activation in the absence of cofactors or activators. Control of PKC activity is regulated through three distinct phosphorylation events. Phosphorylation occurs in vivo at Thr500 in the activation loop, at Thr641 through autophosphorylation, and at the carboxy-terminal hydrophobic site Ser660. Atypical PKC isoforms lack hydrophobic region phosphorylation, which correlates with the presence of glutamic acid rather than the serine or threonine residues found in more typical PKC isoforms. The enzyme PDK1 or a close relative is responsible for PKC activation. A recent addition to the PKC superfamily is PKCu (PKD), which is regulated by DAG and TPA through its C1 domain. PKD is distinguished by the presence of a PH domain and by its unique substrate recognition and Golgi localization. PKC-related kinases (PRK) lack the C1 domain and do not respond to DAG or phorbol esters. Phosphatidylinositol lipids activate PRKs, and small Rho-family GTPases bind to the homology region 1 (HR1) to regulate PRK kinase activity.

NCBI and Uniprot Product Information

• NCBI GI #: 13384594
• NCBI GeneID: 5582
• NCBI Accession #: NP_002730.1
• NCBI GenBank Nucleotide #: NP_002730.1
• UniProt Accession #: P05129
• Molecular Weight: ~ 85kDa
• NCBI Official Full Name: protein kinase C gamma type isoform 2
• NCBI Official Synonym Full Names: protein kinase C gamma
• NCBI Official Symbol: PRKCG
• NCBI Official Synonym Symbols: PKCC; PKCG; SCA14; PKCI(3); PKCgamma; PKC-gamma
• NCBI Protein Information: protein kinase C gamma type
• UniProt Protein Name: Protein kinase C gamma type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCG
• UniProt Synonym Gene Names: PKCG; PKC-gamma
• UniProt Entry Name: KPCG_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play distinct roles in cells. The protein encoded by this gene is one of the PKC family members. This protein kinase is expressed solely in the brain and spinal cord and its localization is restricted to neurons. It has been demonstrated that several neuronal functions, including long term potentiation (LTP) and long term depression (LTD), specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects in this protein have been associated with neurodegenerative disorder spinocerebellar ataxia-14 (SCA14). Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2015]

Uniprot Description

PKCG: a calcium-activated, phospholipid- and diacylglycerol (DAG)-dependent serine/threonine-protein kinase. Expressed in the brain and spinal cord where its localization is restricted to neurons. Several neuronal functions, including long term potentiation and depression (LTP&LTD) specifically require this kinase. Knockout studies in mice also suggest that this kinase may be involved in neuropathic pain development. Defects have been associated with neurodegenerative disorder spinocerebellar ataxia-14. Plays diverse roles in neuronal cells and eye tissues, such as regulation of the neuronal receptors GLUR4 and NMDAR1, modulation of receptors and neuronal functions related to sensitivity to opiates, pain and alcohol, mediation of synaptic function and cell survival after ischemia, and inhibition of gap junction activity after oxidative stress. Binds and phosphorylates GLUR4 glutamate receptor and regulates its function by increasing plasma membrane-associated GRIA4 expression. In primary cerebellar neurons treated with the agonist 3,5-dihyidroxyphenylglycine, functions downstream of the metabotropic glutamate receptor MGLUR5 and phosphorylates NMDAR1 receptor which plays a key role in synaptic plasticity, synaptogenesis, excitotoxicity, memory acquisition and learning. May be involved in the regulation of hippocampal long-term potentiation (LTP), but may be not necessary for the process of synaptic plasticity. May modulate the functionality of mu-type-opioid receptors by participating in a signaling pathway which leads to the phosphorylation and degradation of opioid receptors. May also contributes to chronic morphine-induced changes in nociceptive processing. Plays a role in neuropathic pain mechanisms and contributes to the maintenance of the allodynia pain produced by peripheral inflammation. Plays an important role in initial sensitivity and tolerance to ethanol, by mediating the behavioral effects of ethanol as well as the effects of this drug on the GABA(A) receptors. During and after cerebral ischemia modulate neurotransmission and cell survival in synaptic membranes, and is involved in insulin-induced inhibition of necrosis, an important mechanism for minimizing ischemic injury. Required for the elimination of multiple climbing fibers during innervation of Purkinje cells in developing cerebellum. Is activated in lens epithelial cells upon hydrogen peroxide treatment, and phosphorylates connexin-43, resulting in disassembly of GJA1 gap junction plaques and inhibition of gap junction activity which could provide a protective effect against oxidative stress. Phosphorylates p53 and promotes p53-dependent apoptosis in response to DNA damage. Interacts with GRIA4. Interacts with CDCP1. Interacts with TP53INP1 and p53. Expressed in Purkinje cells of the cerebellar cortex.

Protein type: EC 2.7.11.13; Protein kinase, AGC; Kinase, protein; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 19q13.4

Cellular Component: perinuclear region of cytoplasm; dendrite; plasma membrane; intercellular junction; nucleus; cytosol

Molecular Function: protein kinase C activity; zinc ion binding; protein serine/threonine/tyrosine kinase activity; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: epidermal growth factor receptor signaling pathway; platelet activation; positive regulation of mismatch repair; fibroblast growth factor receptor signaling pathway; nerve growth factor receptor signaling pathway; protein amino acid autophosphorylation; rhythmic process; response to morphine; response to pain; regulation of circadian rhythm; signal transduction; protein amino acid phosphorylation; synaptic transmission; learning and/or memory; phospholipase C activation; innate immune response; chemosensory behavior; negative regulation of protein ubiquitination; negative regulation of neuron apoptosis; blood coagulation; negative regulation of protein catabolic process; phosphorylation; regulation of response to food

Disease: Spinocerebellar Ataxia 14