Rabbit anti-Human GPX1 Polyclonal Antibody | anti-GPX1 antibody

Anti-GPX1 Antibody, Rabbit Polyclonal

Cat. Num. AAA8100704

• Gene Names: GPX1; GPXD; GSHPX1
• Reactivity: Human
• Applications: Western Blot
• Purity: Protein A & Antigen Affinity
• Synonyms: GPX1; Polyclonal Antibody; Anti-GPX1 Antibody; Rabbit Polyclonal; GPX1 Antibody; Rabbit PAb; Antigen Affinity Purified; glutathione peroxidase 1; Anti-GPXD Antibody; Anti-GSHPX1 Antibody; anti-GPX1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human GPX1
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-GPX1 antibody: Western Blot (WB)
• Application Notes: WB: 1:500-1:2000
• Immunogen: A synthetic peptide corresponding to the C-terminus of the human GPX1
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with a synthetic peptide corresponding to the C-terminus of the human GPX1, and purified by antigen affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Western Blot (WB)

(Anti-GPX1 rabbit polyclonal antibody at 1:500 dilution.Lane A: Mouse liver tissue lysate Lysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDaObserved band size:22 kDa)

Related Product Information for anti-GPX1 antibody

GPX1 (Glutathione Peroxidase 1) is a Protein Coding gene. The protein encoded by this gene belongs to the glutathione peroxidase family that catalyzes the reduction of hydrogen peroxide to water. This gene contains an in-frame GCG trinucleotide repeat in the coding region, and three alleles with 4, 5, or 6 repeats have been found in the human population. The allele with 4 GCG repeats has been significantly associated with breast cancer risk in premenopausal women. GPX1 is the most abundant isoform and is expressed in all kidney cells. The GPX1 gene may play a critical role in the development of colorectal cancer. GPx1 played a significant role in regulating mechanical strain-induced POP.

NCBI and Uniprot Product Information

• NCBI GI #: 41406084
• NCBI GeneID: 2876
• NCBI Accession #: NP_000572.2
• NCBI GenBank Nucleotide #: NM_000581.2
• UniProt Accession #: P07203; Q7Z5H1; Q9BW12; E9PAS1
• Molecular Weight: 22,088 Da
• NCBI Official Full Name: glutathione peroxidase 1 isoform 1
• NCBI Official Synonym Full Names: glutathione peroxidase 1
• NCBI Official Symbol: GPX1
• NCBI Official Synonym Symbols: GPXD; GSHPX1
• NCBI Protein Information: glutathione peroxidase 1; GPx-1; GSHPx-1; cellular glutathione peroxidase
• UniProt Protein Name: Glutathione peroxidase 1
• Protein Family: Hydroperoxy fatty acid reductase
• UniProt Gene Name: GPX1
• UniProt Synonym Gene Names: GPx-1; GSHPx-1
• UniProt Entry Name: GPX1_HUMAN

NCBI Description

This gene encodes a member of the glutathione peroxidase family. Glutathione peroxidase functions in the detoxification of hydrogen peroxide, and is one of the most important antioxidant enzymes in humans. This protein is one of only a few proteins known in higher vertebrates to contain selenocysteine, which occurs at the active site of glutathione peroxidase and is coded by UGA, that normally functions as a translation termination codon. In addition, this protein is characterized in a polyalanine sequence polymorphism in the N-terminal region, which includes three alleles with five, six or seven alanine (ALA) repeats in this sequence. The allele with five ALA repeats is significantly associated with breast cancer risk. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

GPX1: Protects the hemoglobin in erythrocytes from oxidative breakdown. Homotetramer. Interacts with MIEN1. Belongs to the glutathione peroxidase family.

Protein type: Other Amino Acids Metabolism - glutathione; EC 1.11.1.9; Lipid Metabolism - arachidonic acid; Oxidoreductase

Chromosomal Location of Human Ortholog: 3p21.3

Cellular Component: mitochondrion; mitochondrial matrix; cytoplasm; nucleus; cytosol

Molecular Function: selenium binding; glutathione binding; phospholipid-hydroperoxide glutathione peroxidase activity; glutathione peroxidase activity; SH3 domain binding

Biological Process: response to nicotine; fat cell differentiation; blood vessel endothelial cell migration; myoblast proliferation; response to toxin; skeletal muscle fiber development; negative regulation of caspase activity; thermoregulation; vasodilation; regulation of gene expression, epigenetic; endothelial cell development; response to estradiol stimulus; regulation of mammary gland epithelial cell proliferation; response to selenium ion; glutathione metabolic process; sensory perception of sound; lipoxygenase pathway; UV protection; response to symbiotic bacterium; hydrogen peroxide catabolic process; response to folic acid; regulation of neuron apoptosis; response to glucose stimulus; response to gamma radiation; arachidonic acid metabolic process; negative regulation of inflammatory response to antigenic stimulus; aging; protein amino acid oxidation; nucleobase, nucleoside and nucleotide metabolic process; skeletal muscle regeneration; angiogenesis involved in wound healing; purine base metabolic process; positive regulation of protein kinase B signaling cascade; response to xenobiotic stimulus; response to reactive oxygen species; triacylglycerol metabolic process; response to hydrogen peroxide; cell redox homeostasis; response to lipid hydroperoxide; heart contraction; purine nucleotide catabolic process; interaction with symbiont; induction of apoptosis by oxidative stress

Disease: Glutathione Peroxidase Deficiency