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Acetyl-Histone H3 Antibody Kit
Acetyl-Histone H3 Antibody Sampler Kit
Applications
Western Blot
Synonyms
Acetyl-Histone H3; Acetyl-Histone H3 Antibody Sampler Kit; Acetyl-Histone H3 antibody kit
Applicable Applications for Acetyl-Histone H3 antibody kit
Western Blot (WB)
Western Blot (WB)
(Western Blot analysis of MCF7 cells using Histone H3 Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Rat muscle, kindey using Acetyl-Histone H3 (Lys9) Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of 3T3 cells with Histone H3 (Acetyl Lys14) Polyclonal Antibody.)
Western Blot (WB)
(Western Blot analysis of L929 cells using Histone H3 (Acetyl Lys23) Polyclonal Antibody at dilution of 1:1000.)
Western Blot (WB)
(Western Blot analysis of Hela cells using Histone H3 (Acetyl Lys27) Polyclonal Antibody at dilution of 1:2000.)
Western Blot (WB)
(Western Blot analysis of Hela cells untreated (-) or treated using Acetyl-Histone H3 (Lys56) Polyclonal Antibody at dilution of 1:20000.)
Related Product Information for Acetyl-Histone H3 antibody kit
Background: Modulation of chromatin structure plays an important role in the regulation of transcription in eukaryotes. The nucleosome, made up of DNA wound around eight core histone proteins (two each of H2A, H2B, H3, and H4), is the primary building block of chromatin. The amino- terminal tails of core histones undergo various post-translational modifications, including acetylation, phosphorylation, methylation, and ubiquitination. These modifications occur in response to various stimuli and have a direct effect on the accessibility of chromatin to transcription factors and, therefore, gene expression. In most species, histone H2B is primarily acetylated at Lys5, 12, 15, and 20. Histone H3 is primarily acetylated at Lys9, 14, 18, 23, 27, and 56. Acetylation of H3 at Lys9 appears to have a dominant role in histone deposition and chromatin assembly in some organisms. Phosphorylation at Ser10, Ser28, and Thr11 of histone H3 is tightly correlated with chromosome condensation during both mitosis and meiosis. Phosphorylation at Thr3 of histone H3 is highly conserved among many species and is catalyzed by the kinase haspin. Immunostaining with phospho-specific antibodies in mammalian cells reveals mitotic phosphorylation at Thr3 of H3 in prophase and its dephosphorylation during anaphase.
