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Western Blot (WB) (Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.)

HSP27 antibody kit

HSP27 Antibody Sampler Kit

Applications
Western Blot
Synonyms
HSP27; HSP27 Antibody Sampler Kit; HSP27 antibody kit
Ordering
For Research Use Only!
Applicable Applications for HSP27 antibody kit
Western Blot (WB)

Western Blot (WB)

(Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB) (Western Blot analysis of 22RV1 cells using HSP27 Polyclonal Antibody at dilution of 1:1000.)

Western Blot (WB)

(Western Blot analysis of COS7 cells with Phospho-HSP27 (Ser15) Polyclonal Antibody)

Western Blot (WB) (Western Blot analysis of COS7 cells with Phospho-HSP27 (Ser15) Polyclonal Antibody)

Western Blot (WB)

(Western Blot analysis of Hela cells with Phospho-HSP27 (Ser78) Polyclonal Antibody.)

Western Blot (WB) (Western Blot analysis of Hela cells with Phospho-HSP27 (Ser78) Polyclonal Antibody.)

Western Blot (WB)

(Western Blot analysis of HuvEc cells with Phospho-HSP27 (Ser82) Polyclonal Antibody at dilution of 1:1000)

Western Blot (WB) (Western Blot analysis of HuvEc cells with Phospho-HSP27 (Ser82) Polyclonal Antibody at dilution of 1:1000)
Related Product Information for HSP27 antibody kit
Background: Heat shock protein (HSP) 27 is one of the small HSPs that are constitutively expressed at different levels in various cell types and tissues. Like other small HSPs, HSP27 is regulated at both the transcriptional and posttranslational levels. In response to stress, the HSP27 expression increases several-fold to confer cellular resistance to the adverse environmental change. HSP27 is phosphorylated at Ser15, Ser78, and Ser82 by MAPKAPK-2 as a result of the activation of the p38 MAP kinase pathway. Phosphorylation of HSP27 causes a change in its tertiary structure, which shifts from large homotypic multimers to dimers and monomers. It has been shown that phosphorylation and increased concentration of HSP27 modulates actin polymerization and reorganization.

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Product Notes

The HSP27 (Catalog #AAA2550049) is an Antibody Kit and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's HSP27 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the HSP27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "HSP27, Antibody Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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